1kfa
From Proteopedia
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|PDB= 1kfa |SIZE=350|CAPTION= <scene name='initialview01'>1kfa</scene>, resolution 2.80Å | |PDB= 1kfa |SIZE=350|CAPTION= <scene name='initialview01'>1kfa</scene>, resolution 2.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=GA4:GIBBERELLIN A4'>GA4</scene> | + | |LIGAND= <scene name='pdbligand=GA4:GIBBERELLIN+A4'>GA4</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kfa OCA], [http://www.ebi.ac.uk/pdbsum/1kfa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kfa RCSB]</span> | ||
}} | }} | ||
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[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
| - | [[Category: GA4]] | ||
[[Category: immunoglobuiln fold]] | [[Category: immunoglobuiln fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:47:45 2008'' |
Revision as of 18:47, 30 March 2008
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| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Fab fragment complexed with gibberellin A4
Overview
Gibberellins, a class of plant hormones, consist of more than 120 members. Only a few of them are recognized by a receptor that remains unknown. The haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody and GA(4). We carried out an X-ray crystallographic analysis of the 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by using the molecular replacement method. The crystal structure of the Fab fragment showed the typical immunoglobulin fold of the beta-barrel structure which is the common motif of all antibodies. A small hapten-combining site was made up of three heavy chain CDR loops. On the other hand, CDRs of the light chain did not interact directly with GA(4). The C/D rings of the GA(4) molecule were in van der Waals contact mainly with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3 beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
About this Structure
1KFA is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:12054627
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