1kfm
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1eq7|1EQ7]], [[1jcb|1JCB]], [[1kfn|1KFN]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kfm OCA], [http://www.ebi.ac.uk/pdbsum/1kfm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kfm RCSB]</span> | ||
}} | }} | ||
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[[Category: protein folding]] | [[Category: protein folding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:47:53 2008'' |
Revision as of 18:47, 30 March 2008
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| , resolution 2.00Å | |||||||
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| Related: | 1EQ7, 1JCB, 1KFN
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Core side-chain packing and backbone conformation in Lpp-56 coiled-coil mutants
Overview
Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stability of the Lpp-56 trimerization domain. The X-ray crystal structures of Lpp-56 mutants with alanine substitutions at two and four interior core positions reveal trimeric coiled coils in which the twist of individual helices and the helix-helix spacing vary significantly to achieve the most favored superhelical packing arrangement. Introduction of each alanine "layer" into the hydrophobic core destabilizes the superhelix by 1.4 kcal mol(-1). Although the methyl groups of the alanine residues pack at their optimum van der Waals contacts in the coiled-coil trimer, they provide a smaller component of hydrophobic interactions than bulky hydrophobic side-chains to the thermodynamic stability. Thus, specific side-chain packing in the hydrophobic core of coiled coils are important determinants of protein main-chain conformation and stability.
About this Structure
1KFM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Core side-chain packing and backbone conformation in Lpp-56 coiled-coil mutants., Liu J, Cao W, Lu M, J Mol Biol. 2002 May 3;318(3):877-88. PMID:12054830
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