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4aub

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Revision as of 07:54, 4 August 2016

the complex Structure of the bacterial aldo-keto reductase AKR14A1 with NADP and citrate

Structural highlights

4aub is a 8 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4ast
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GPR_ECOLI] Catalyzes the stereospecific, NADPH-dependent reduction of L-glyceraldehyde 3-phosphate (L-GAP). The physiological role of gpr is the detoxification of L-GAP, which may be formed by non-enzymatic racemization of GAP. Also involved in the stress response as a methylglyoxal reductase which converts the toxic metabolite methylglyoxal to acetol in vitro and in vivo.^[1] ^[2] ^[3]

Publication Abstract from PubMed

The genome of Escherichia coli K12 contains 9 open reading frames encoding aldo/keto reductases (AKRs) that are differentially regulated and sequence diverse. A significant amount of data is available for the E. coli AKRs through the availability of gene knockouts and gene expression studies, which adds to the biochemical and kinetic data. This together with the availability of crystal structures for nearly half of the E. coli AKRs and homologues of several others provides an opportunity to look at the diversity of these representative bacterial AKRs. Based around the common AKR fold of (beta/alpha)8 barrel with two additional alpha-helices, the E. coli AKRs have a loop structure that is more diverse than their mammalian counterparts, creating a variety of active site architectures. Nearly half of the AKRs are expected to be monomeric, but there are examples of dimeric, trimeric and octameric enzymes, as well as diversity in specificity for NAD as well as NADP as a cofactor. However in functional assignments and characterisation of enzyme activities there is a paucity of data when compared to the mammalian AKR enzymes.

The diversity of microbial aldo/keto reductases from Escherichia coli K12.,Lapthorn AJ, Zhu X, Ellis EM Chem Biol Interact. 2013 Feb 25;202(1-3):168-77. doi: 10.1016/j.cbi.2012.10.008. , Epub 2012 Oct 24. PMID:23103600^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grant AW, Steel G, Waugh H, Ellis EM. A novel aldo-keto reductase from Escherichia coli can increase resistance to methylglyoxal toxicity. FEMS Microbiol Lett. 2003 Jan 21;218(1):93-9. PMID:12583903
↑ Ko J, Kim I, Yoo S, Min B, Kim K, Park C. Conversion of methylglyoxal to acetol by Escherichia coli aldo-keto reductases. J Bacteriol. 2005 Aug;187(16):5782-9. PMID:16077126 doi:http://dx.doi.org/10.1128/JB.187.16.5782-5789.2005
↑ Desai KK, Miller BG. A metabolic bypass of the triosephosphate isomerase reaction. Biochemistry. 2008 Aug 5;47(31):7983-5. doi: 10.1021/bi801054v. Epub 2008 Jul 12. PMID:18620424 doi:http://dx.doi.org/10.1021/bi801054v
↑ Lapthorn AJ, Zhu X, Ellis EM. The diversity of microbial aldo/keto reductases from Escherichia coli K12. Chem Biol Interact. 2013 Feb 25;202(1-3):168-77. doi: 10.1016/j.cbi.2012.10.008. , Epub 2012 Oct 24. PMID:23103600 doi:http://dx.doi.org/10.1016/j.cbi.2012.10.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4aub"

Categories: Ecoli | Ellis, E M | Lapthorn, A | Zhu, X | Oxidoreductase

@@ Line 1: / Line 1: @@
 ==the complex Structure of the bacterial aldo-keto reductase AKR14A1 with NADP and citrate==
 <StructureSection load='4aub' size='340' side='right' caption='[[4aub]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ast|4ast]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aub OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aub RCSB], [http://www.ebi.ac.uk/pdbsum/4aub PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aub OCA], [http://pdbe.org/4aub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4aub RCSB], [http://www.ebi.ac.uk/pdbsum/4aub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4aub ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4aub" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4aub

From Proteopedia

Revision as of 07:54, 4 August 2016

the complex Structure of the bacterial aldo-keto reductase AKR14A1 with NADP and citrate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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