1kne
From Proteopedia
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|PDB= 1kne |SIZE=350|CAPTION= <scene name='initialview01'>1kne</scene>, resolution 2.40Å | |PDB= 1kne |SIZE=350|CAPTION= <scene name='initialview01'>1kne</scene>, resolution 2.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kna|1KNA]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kne OCA], [http://www.ebi.ac.uk/pdbsum/1kne PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kne RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: trimethyllysine]] | [[Category: trimethyllysine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:50:57 2008'' |
Revision as of 18:50, 30 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | |||||||
| Related: | 1KNA
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9
Overview
The chromodomain of the HP1 family of proteins recognizes histone tails with specifically methylated lysines. Here, we present structural, energetic, and mutational analyses of the complex between the Drosophila HP1 chromodomain and the histone H3 tail with a methyllysine at residue 9, a modification associated with epigenetic silencing. The histone tail inserts as a beta strand, completing the beta-sandwich architecture of the chromodomain. The methylammonium group is caged by three aromatic side chains, whereas adjacent residues form discerning contacts with one face of the chromodomain. Comparison of dimethyl- and trimethyllysine-containing complexes suggests a role for cation-pi and van der Waals interactions, with trimethylation slightly improving the binding affinity.
About this Structure
1KNE is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail., Jacobs SA, Khorasanizadeh S, Science. 2002 Mar 15;295(5562):2080-3. Epub 2002 Feb 21. PMID:11859155
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