4akg

From Proteopedia

(Difference between revisions)

Revision as of 09:59, 4 August 2016

Dynein Motor Domain - ATP complex

Structural highlights

4akg is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	1b8x, 1dug, 1gne, 1gta, 1gtb, 1m99, 1m9a, 1m9b, 1u87, 1u88, 1ua5, 1y6e, 4ai6, 4akh, 4aki
Activity:	Glutathione transferase, with EC number 2.5.1.18
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GST26_SCHJA] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Publication Abstract from PubMed

Dyneins power the beating of cilia and flagella, transport various intracellular cargos and are necessary for mitosis. All dyneins have a approximately 300-kDa motor domain consisting of a ring of six AAA+ domains. ATP hydrolysis in the AAA+ ring drives the cyclic relocation of a motile element, the linker domain, to generate the force necessary for movement. How the linker interacts with the ring during the ATP hydrolysis cycle is not known. Here we present a 3.3-A crystal structure of the motor domain of Saccharomyces cerevisiae cytoplasmic dynein, crystallized in the absence of nucleotides. The linker is docked to a conserved site on AAA5, which is confirmed by mutagenesis as functionally necessary. Nucleotide soaking experiments show that the main ATP hydrolysis site in dynein (AAA1) is in a low-nucleotide affinity conformation and reveal the nucleotide interactions of the other three sites (AAA2, AAA3 and AAA4).

Insights into dynein motor domain function from a 3.3-A crystal structure.,Schmidt H, Gleave ES, Carter AP Nat Struct Mol Biol. 2012 Mar 14;19(5):492-7. doi: 10.1038/nsmb.2272. PMID:22426545^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schmidt H, Gleave ES, Carter AP. Insights into dynein motor domain function from a 3.3-A crystal structure. Nat Struct Mol Biol. 2012 Mar 14;19(5):492-7. doi: 10.1038/nsmb.2272. PMID:22426545 doi:10.1038/nsmb.2272

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4akg"

@@ Line 1: / Line 1: @@
 ==Dynein Motor Domain - ATP complex==
 <StructureSection load='4akg' size='340' side='right' caption='[[4akg]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4akg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_japonicum,_saccharomyces_cerevisiae Schistosoma japonicum, saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4akg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AKG FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b8x|1b8x]], [[1dug|1dug]], [[1gne|1gne]], [[1gta|1gta]], [[1gtb|1gtb]], [[1m99|1m99]], [[1m9a|1m9a]], [[1m9b|1m9b]], [[1u87|1u87]], [[1u88|1u88]], [[1ua5|1ua5]], [[1y6e|1y6e]], [[4ai6|4ai6]], [[4akh|4akh]], [[4aki|4aki]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4akg RCSB], [http://www.ebi.ac.uk/pdbsum/4akg PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4akg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akg OCA], [http://pdbe.org/4akg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4akg RCSB], [http://www.ebi.ac.uk/pdbsum/4akg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4akg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4akg" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 27: / Line 29: @@
 </StructureSection>
 [[Category: Glutathione transferase]]
-[[Category: Schistosoma japonicum, saccharomyces cerevisiae]]
 [[Category: Carter, A P]]
 [[Category: Gleave, E S]]

4akg

From Proteopedia

Revision as of 09:59, 4 August 2016

Dynein Motor Domain - ATP complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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