1kou
From Proteopedia
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|PDB= 1kou |SIZE=350|CAPTION= <scene name='initialview01'>1kou</scene>, resolution 1.16Å | |PDB= 1kou |SIZE=350|CAPTION= <scene name='initialview01'>1kou</scene>, resolution 1.16Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=DHC:CAFFEIC+ACID'>DHC</scene> | + | |LIGAND= <scene name='pdbligand=DHC:CAFFEIC+ACID'>DHC</scene>, <scene name='pdbligand=NBU:N-BUTANE'>NBU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | |GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1d7e|1D7E]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kou OCA], [http://www.ebi.ac.uk/pdbsum/1kou PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kou RCSB]</span> | ||
}} | }} | ||
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[[Category: Hellingwerf, K J.]] | [[Category: Hellingwerf, K J.]] | ||
[[Category: Joshua-Tor, L.]] | [[Category: Joshua-Tor, L.]] | ||
| - | [[Category: DHC]] | ||
| - | [[Category: NBU]] | ||
[[Category: photoreceptor]] | [[Category: photoreceptor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:30 2008'' |
Revision as of 18:51, 30 March 2008
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| , resolution 1.16Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | PYP (Halorhodospira halophila) | ||||||
| Related: | 1D7E
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution
Overview
A structural study is described of the photoactive yellow protein (PYP) reconstituted with the chromophore derivative 3,4-dihydroxycinnamic acid. The crystal structure of PYP reconstituted with this chromophore at 1.16 A resolution is reported in space group P6(5). This is the first high-resolution structure of a photoreceptor containing a modified chromophore. The introduction of an extra hydroxyl group in the native chromophore (i.e. p-coumaric acid) appears to perturb the structure of the hybrid yellow protein only slightly. The chromophore is bound by the protein in two different conformations, separated by a rotation of 180 degrees of the catechol ring. In combination with available spectroscopic data, it is concluded that the caffeic acid chromophore binds to the protein in a strained conformation, which leads to a faster ejection from the chromophore-binding pocket upon pB formation.
About this Structure
1KOU is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution., van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:11914481
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