4chk

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(Difference between revisions)

Revision as of 10:26, 4 August 2016

Crystal Structure of the ARF5 oligomerization domain

Structural highlights

4chk is a 8 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARFE_ARATH] Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity.^[1] ^[2] ^[3]

Publication Abstract from PubMed

The plant hormone auxin is a key morphogenetic regulator acting from embryogenesis onwards. Transcriptional events in response to auxin are mediated by the auxin response factor (ARF) transcription factors and the Aux/IAA (IAA) transcriptional repressors. At low auxin concentrations, IAA repressors associate with ARF proteins and recruit corepressors that prevent auxin-induced gene expression. At higher auxin concentrations, IAAs are degraded and ARFs become free to regulate auxin-responsive genes. The interaction between ARFs and IAAs is thus central to auxin signalling and occurs through the highly conserved domain III/IV present in both types of proteins. Here, we report the crystal structure of ARF5 domain III/IV and reveal the molecular determinants of ARF-IAA interactions. We further provide evidence that ARFs have the potential to oligomerize, a property that could be important for gene regulation in response to auxin.

Structural basis for oligomerization of auxin transcriptional regulators.,Nanao MH, Vinos-Poyo T, Brunoud G, Thevenon E, Mazzoleni M, Mast D, Laine S, Wang S, Hagen G, Li H, Guilfoyle TJ, Parcy F, Vernoux T, Dumas R Nat Commun. 2014 Apr 7;5:3617. doi: 10.1038/ncomms4617. PMID:24710426^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hagen G, Guilfoyle T. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol Biol. 2002 Jun-Jul;49(3-4):373-85. PMID:12036261
↑ Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T. Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4. Development. 2004 Mar;131(5):1089-100. PMID:14973283 doi:http://dx.doi.org/10.1242/dev.00925
↑ Vidaurre DP, Ploense S, Krogan NT, Berleth T. AMP1 and MP antagonistically regulate embryo and meristem development in Arabidopsis. Development. 2007 Jul;134(14):2561-7. Epub 2007 Jun 6. PMID:17553903 doi:http://dx.doi.org/10.1242/dev.006759
↑ Nanao MH, Vinos-Poyo T, Brunoud G, Thevenon E, Mazzoleni M, Mast D, Laine S, Wang S, Hagen G, Li H, Guilfoyle TJ, Parcy F, Vernoux T, Dumas R. Structural basis for oligomerization of auxin transcriptional regulators. Nat Commun. 2014 Apr 7;5:3617. doi: 10.1038/ncomms4617. PMID:24710426 doi:http://dx.doi.org/10.1038/ncomms4617

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4chk"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the ARF5 oligomerization domain==
 <StructureSection load='4chk' size='340' side='right' caption='[[4chk]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4chk]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CHK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4chk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4chk RCSB], [http://www.ebi.ac.uk/pdbsum/4chk PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4chk OCA], [http://pdbe.org/4chk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4chk RCSB], [http://www.ebi.ac.uk/pdbsum/4chk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4chk ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4chk" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4chk

From Proteopedia

Revision as of 10:26, 4 August 2016

Crystal Structure of the ARF5 oligomerization domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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