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| | ==Crystal structure of Kap121p bound to RanGTP== | | ==Crystal structure of Kap121p bound to RanGTP== |
| | <StructureSection load='3w3z' size='340' side='right' caption='[[3w3z]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='3w3z' size='340' side='right' caption='[[3w3z]], [[Resolution|resolution]] 2.70Å' scene=''> |
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| | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w3t|3w3t]], [[3w3u|3w3u]], [[3w3v|3w3v]], [[3w3w|3w3w]], [[3w3x|3w3x]], [[3w3y|3w3y]]</td></tr> | | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w3t|3w3t]], [[3w3u|3w3u]], [[3w3v|3w3v]], [[3w3w|3w3w]], [[3w3x|3w3x]], [[3w3y|3w3y]]</td></tr> |
| | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PSE1, KAP121, YMR308C, YM9952.10C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), RAN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 CANFA])</td></tr> | | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PSE1, KAP121, YMR308C, YM9952.10C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), RAN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 CANFA])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w3z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w3z RCSB], [http://www.ebi.ac.uk/pdbsum/3w3z PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w3z OCA], [http://pdbe.org/3w3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w3z RCSB], [http://www.ebi.ac.uk/pdbsum/3w3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w3z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3w3z" style="background-color:#fffaf0;"></div> |
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| | ==See Also== | | ==See Also== |
| | *[[GTP-binding protein|GTP-binding protein]] | | *[[GTP-binding protein|GTP-binding protein]] |
| | + | *[[Importin|Importin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Structural highlights
3w3z is a 2 chain structure with sequence from Baker's yeast and Canfa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , |
| NonStd Res: | |
| Related: | 3w3t, 3w3u, 3w3v, 3w3w, 3w3x, 3w3y |
| Gene: | PSE1, KAP121, YMR308C, YM9952.10C (Baker's yeast), RAN (CANFA) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[IMB3_YEAST] Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). Plays a role in protein secretion. [RAN_CANFA] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (By similarity).
Publication Abstract from PubMed
Kap121p (also known as Pse1p) is an essential karyopherin that mediates nuclear import of a plethora of cargoes including cell-cycle regulators, transcription factors, and ribosomal proteins in Saccharomyces cerevisiae. It has been proposed that the spindle assembly checkpoint signaling triggers molecular rearrangements of nuclear pore complexes and thereby arrests Kap121p-mediated nuclear import at metaphase, while leaving import mediated by other karyopherins unaffected. The Kap121p-specific import inhibition is required for normal progression through mitosis. To understand the structural basis for Kap121p-mediated nuclear import and its unique regulatory mechanism during mitosis, we determined crystal structures of Kap121p in isolation and also in complex with either its import cargoes or nucleoporin Nup53p or RanGTP. Kap121p has a superhelical structure composed of 24 HEAT repeats. The structures of Kap121p-cargo complexes define a non-conventional nuclear localization signal (NLS) that has a consensus sequence of KV/IxKx1-2K/H/R. The structure of Kap121p-Nup53p complex shows that cargo and Nup53p compete for the same high-affinity binding site, explaining how Nup53p binding forces cargo release when the Kap121p binding site of Nup53p is exposed during mitosis. Comparison of the NLS- and RanGTP-complexes reveals that RanGTP binding not only occludes the cargo-binding site but also forces Kap121p into a conformation that is incompatible with NLS recognition.
Structural basis for cell-cycle dependent nuclear import mediated by the karyopherin Kap121p.,Kobayashi J, Matsuura Y J Mol Biol. 2013 Mar 27. pii: S0022-2836(13)00194-0. doi:, 10.1016/j.jmb.2013.02.035. PMID:23541588[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kobayashi J, Matsuura Y. Structural basis for cell-cycle dependent nuclear import mediated by the karyopherin Kap121p. J Mol Biol. 2013 Mar 27. pii: S0022-2836(13)00194-0. doi:, 10.1016/j.jmb.2013.02.035. PMID:23541588 doi:http://dx.doi.org/10.1016/j.jmb.2013.02.035
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