1kpi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 8: Line 8:
|GENE= cmaA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
|GENE= cmaA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08242 Methyltransf_12], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02353 CMAS], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2242 CobL]</span>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08242 Methyltransf_12], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02353 CMAS], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2242 CobL]</span>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kpi OCA], [http://www.ebi.ac.uk/pdbsum/1kpi PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1kpi RCSB]</span>
+
|RELATEDENTRY=[[1kp9|1KP9]], [[1kpg|1KPG]], [[1kph|1KPH]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kpi OCA], [http://www.ebi.ac.uk/pdbsum/1kpi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kpi RCSB]</span>
}}
}}
Line 38: Line 39:
[[Category: tbsgc]]
[[Category: tbsgc]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:55:15 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:45 2008''

Revision as of 18:51, 30 March 2008

Image:1kpi.gif


PDB ID 1kpi

Drag the structure with the mouse to rotate
, resolution 2.65Å
Ligands: , , ,
Gene: cmaA2 (Mycobacterium tuberculosis)
Activity: Cyclopropane-fatty-acyl-phospholipid synthase, with EC number 2.1.1.79
Domains: Methyltransf_12, CMAS, CobL
Related: 1KP9, 1KPG, 1KPH


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of mycolic acid cyclopropane synthase CmaA2 complexed with SAH and DDDMAB


Overview

Mycolic acids are major components of the cell wall of Mycobacterium tuberculosis. Several studies indicate that functional groups in the acyl chain of mycolic acids are important for pathogenesis and persistence. There are at least three mycolic acid cyclopropane synthases (PcaA, CmaA1, and CmaA2) that are responsible for these site-specific modifications of mycolic acids. To derive information on the specificity and enzyme mechanism of the family of proteins, the crystal structures of CmaA1, CmaA2, and PcaA were solved to 2-, 2-, and 2.65-A resolution, respectively. All three enzymes have a seven-stranded alpha/beta fold similar to other methyltransferases with the location and interactions with the cofactor S-adenosyl-l-methionine conserved. The structures of the ternary complexes demonstrate the position of the mycolic acid substrate binding site. Close examination of the active site reveals electron density that we believe represents a bicarbonate ion. The structures support the hypothesis that these enzymes catalyze methyl transfer via a carbocation mechanism in which the bicarbonate ion acts as a general base. In addition, comparison of the enzyme structures reveals a possible mechanism for substrate specificity. These structures provide a foundation for rational-drug design, which may lead to the development of new inhibitors effective against persistent bacteria.

About this Structure

1KPI is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis., Huang CC, Smith CV, Glickman MS, Jacobs WR Jr, Sacchettini JC, J Biol Chem. 2002 Mar 29;277(13):11559-69. Epub 2001 Dec 26. PMID:11756461

Page seeded by OCA on Sun Mar 30 21:51:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kpi"
Personal tools