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4hpd

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Revision as of 11:02, 4 August 2016

Crystal structure of Nitrophorin 4 from Rhodnius prolixus Complexed with homocysteine at pH 7.4

Structural highlights

4hpd is a 1 chain structure with sequence from Rhopr. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4hpa, 4hpb, 4hpc
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NP4_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).

Publication Abstract from PubMed

Nitrophorins are proteins occurring in the saliva of the blood-sucking insect Rhodnius prolixus to carry NO as a vasodilator and blood-coagulation inhibitor into the victim's tissue. It was suggested that the rate of NO release can be enhanced by the blood-plasma component L-cysteine [J.M.C.Ribeiro, Insect Biochem. Mol. Biol. 26 (1996) 899-905]. However, the mechanism of the reaction is not clear. In the attempt to exploit the reaction in detail, complexes of nitrophorin 4 (NP4) with the thiols 2-mercaptoethanol, L-cysteine, and L-homocysteine and with HS(-) were formed and characterized under anaerobic conditions using absorption spectroscopy, X-ray crystallography, and EPR spectroscopy. In contrast to met-myoglobin, which is reduced by L-cysteine, all four compounds form low-spin Fe(III) complexes with NP4. The weak equilibration constants (167-5200 M(-1)) neither support significant complexation nor the simple displacement of NO in vivo. Both amino acid based thiols form additional H-bonds with side chains of the heme pocket entry. Glutathione and L-methionine did not form a complex, indicating the specificity of the complexes with L-cysteine and L-homocysteine. Continuous wave EPR spectroscopy reveals the simultaneous existence of three low-spin systems in each case that are attributed to various protonation and/or conformational stages in the heme pocket. Electron nuclear double resonance (ENDOR) spectroscopy demonstrates that the thiol sulfurs are, at least in part, protonated. Overall, the results not only demonstrate the good accessibility of the NP4 heme center by biologically relevant thiols, but also represent the first structural characterization of a ferriheme protein in complex with L-cysteine L-homocysteine.

Complexes of ferriheme nitrophorin 4 with low-molecular weight thiol(ate)s occurring in blood plasma.,He C, Nishikawa K, Erdem OF, Reijerse E, Ogata H, Lubitz W, Knipp M J Inorg Biochem. 2013 May;122:38-48. doi: 10.1016/j.jinorgbio.2013.01.012. Epub, 2013 Feb 4. PMID:23474537^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ He C, Nishikawa K, Erdem OF, Reijerse E, Ogata H, Lubitz W, Knipp M. Complexes of ferriheme nitrophorin 4 with low-molecular weight thiol(ate)s occurring in blood plasma. J Inorg Biochem. 2013 May;122:38-48. doi: 10.1016/j.jinorgbio.2013.01.012. Epub, 2013 Feb 4. PMID:23474537 doi:http://dx.doi.org/10.1016/j.jinorgbio.2013.01.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hpd"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Nitrophorin 4 from Rhodnius prolixus Complexed with homocysteine at pH 7.4==
 <StructureSection load='4hpd' size='340' side='right' caption='[[4hpd]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hpa|4hpa]], [[4hpb|4hpb]], [[4hpc|4hpc]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpd RCSB], [http://www.ebi.ac.uk/pdbsum/4hpd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpd OCA], [http://pdbe.org/4hpd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hpd RCSB], [http://www.ebi.ac.uk/pdbsum/4hpd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4hpd" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4hpd

From Proteopedia

Revision as of 11:02, 4 August 2016

Crystal structure of Nitrophorin 4 from Rhodnius prolixus Complexed with homocysteine at pH 7.4

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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