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4m64

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Revision as of 11:11, 4 August 2016

3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium

Structural highlights

4m64 is a 4 chain structure with sequence from Salty. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	melB, STM4299 (SALTY)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MELB_SALTY] Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).

Publication Abstract from PubMed

The bacterial melibiose permease (MelB) belongs to the glycoside-pentoside-hexuronide:cation symporter family, a part of the major facilitator superfamily (MFS). Structural information regarding glycoside-pentoside-hexuronide:cation symporter family transporters and other Na(+)-coupled permeases within MFS has been lacking, although a wealth of biochemical and biophysical data are available. Here we present the three-dimensional crystal structures of Salmonella typhimurium MelBSt in two conformations, representing an outward partially occluded and an outward inactive state of MelBSt. MelB adopts a typical MFS fold and contains a previously unidentified cation-binding motif. Three conserved acidic residues form a pyramidal-shaped cation-binding site for Na(+), Li(+) or H(+), which is in close proximity to the sugar-binding site. Both cosubstrate-binding sites are mainly contributed by the residues from the amino-terminal domain. These two structures and the functional data presented here provide mechanistic insights into Na(+)/melibiose symport. We also postulate a structural foundation for the conformational cycling necessary for transport catalysed by MFS permeases in general.

Structure-based mechanism for Na(+)/melibiose symport by MelB.,Ethayathulla AS, Yousef MS, Amin A, Leblanc G, Kaback HR, Guan L Nat Commun. 2014 Jan 6;5:3009. doi: 10.1038/ncomms4009. PMID:24389923^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ethayathulla AS, Yousef MS, Amin A, Leblanc G, Kaback HR, Guan L. Structure-based mechanism for Na(+)/melibiose symport by MelB. Nat Commun. 2014 Jan 6;5:3009. doi: 10.1038/ncomms4009. PMID:24389923 doi:http://dx.doi.org/10.1038/ncomms4009

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4m64"

@@ Line 1: / Line 1: @@
 ==3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium==
 <StructureSection load='4m64' size='340' side='right' caption='[[4m64]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4m64]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M64 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M64 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">melB, STM4299 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m64 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m64 RCSB], [http://www.ebi.ac.uk/pdbsum/4m64 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m64 OCA], [http://pdbe.org/4m64 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m64 RCSB], [http://www.ebi.ac.uk/pdbsum/4m64 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m64 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4m64" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4m64

From Proteopedia

Revision as of 11:11, 4 August 2016

3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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