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3t2v

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Revision as of 11:24, 4 August 2016

Crystal structure of the complex of peptidoglycan recognition protein-short (CPGRP-S) with mycolic acid at 2.5 A resolution

Structural highlights

3t2v is a 4 chain structure with sequence from Camelus dromedarius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PGRP1_CAMDR] Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. Is microbicidal for Gram-positive and Gram-negative bacteria and yeast. May function in intracellular killing of bacteria (By similarity).

Publication Abstract from PubMed

Short peptidoglycan recognition protein (PGRP-S) is a member of the mammalian innate immune system. PGRP-S from Camelus dromedarius (CPGRP-S) has been shown to bind to lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN). Its structure consists of four molecules A, B, C and D with ligand binding clefts situated at A-B and C-D contacts. It has been shown that LPS, LTA and PGN bind to CPGRP-S at C-D contact. The cleft at the A-B contact indicated features that suggested a possible binding of fatty acids including mycolic acid of Mycobacterium tuberculosis. Therefore, binding studies of CPGRP-S were carried out with fatty acids, butyric acid, lauric acid, myristic acid, stearic acid and mycolic acid which showed affinities in the range of 10(-5) to 10(-8) M. Structure determinations of the complexes of CPGRP-S with above fatty acids showed that they bound to CPGRP-S in the cleft at the A-B contact. The flow cytometric studies showed that mycolic acid induced the production of pro-inflammatory cytokines, TNF-alpha and IFN-gamma by CD3+ T cells. The concentrations of cytokines increased considerably with increasing concentrations of mycolic acid. However, their levels decreased substantially on adding CPGRP-S.

Structural basis of the binding of fatty acids to peptidoglycan recognition protein, PGRP-S through second binding site.,Sharma P, Yamini S, Dube D, Singh A, Mal G, Pandey N, Sinha M, Singh AK, Dey S, Kaur P, Mitra DK, Sharma S, Singh TP Arch Biochem Biophys. 2013 Jan 1;529(1):1-10. doi: 10.1016/j.abb.2012.11.001., Epub 2012 Nov 10. PMID:23149273^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sharma P, Yamini S, Dube D, Singh A, Mal G, Pandey N, Sinha M, Singh AK, Dey S, Kaur P, Mitra DK, Sharma S, Singh TP. Structural basis of the binding of fatty acids to peptidoglycan recognition protein, PGRP-S through second binding site. Arch Biochem Biophys. 2013 Jan 1;529(1):1-10. doi: 10.1016/j.abb.2012.11.001., Epub 2012 Nov 10. PMID:23149273 doi:10.1016/j.abb.2012.11.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3t2v"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the complex of peptidoglycan recognition protein-short (CPGRP-S) with mycolic acid at 2.5 A resolution==
 <StructureSection load='3t2v' size='340' side='right' caption='[[3t2v]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3t2v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T2V FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KKJ:(2S,3R)-2-HEXYL-3-HYDROXYNONANOIC+ACID'>KKJ</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t2v RCSB], [http://www.ebi.ac.uk/pdbsum/3t2v PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2v OCA], [http://pdbe.org/3t2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t2v RCSB], [http://www.ebi.ac.uk/pdbsum/3t2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t2v ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3t2v" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3t2v

From Proteopedia

Revision as of 11:24, 4 August 2016

Crystal structure of the complex of peptidoglycan recognition protein-short (CPGRP-S) with mycolic acid at 2.5 A resolution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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