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2wtb

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Revision as of 11:41, 4 August 2016

ARABIDOPSIS THALIANA MULTIFUCTIONAL PROTEIN, MFP2

Structural highlights

2wtb is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MFP2_ARATH] Involved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18).^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Plant fatty acids can be completely degraded within the peroxisomes. Fatty acid degradation plays a role in several plant processes including plant hormone synthesis and seed germination. Two multifunctional peroxisomal isozymes, MFP2 and AIM1, both with 2-trans-enoyl-CoA hydratase and l-3-hydroxyacyl-CoA dehydrogenase activities, function in mouse ear cress (Arabidopsis thaliana) peroxisomal beta-oxidation, where fatty acids are degraded by the sequential removal of two carbon units. A deficiency in either of the two isozymes gives rise to a different phenotype; the biochemical and molecular background for these differences is not known. Structure determination of Arabidopsis MFP2 revealed that plant peroxisomal MFPs can be grouped into two families, as defined by a specific pattern of amino acid residues in the flexible loop of the acyl-binding pocket of the 2-trans-enoyl-CoA hydratase domain. This could explain the differences in substrate preferences and specific biological functions of the two isozymes. The in vitro substrate preference profiles illustrate that the Arabidopsis AIM1 hydratase has a preference for short chain acyl-CoAs compared with the Arabidopsis MFP2 hydratase. Remarkably, neither of the two was able to catabolize enoyl-CoA substrates longer than 14 carbon atoms efficiently, suggesting the existence of an uncharacterized long chain enoyl-CoA hydratase in Arabidopsis peroxisomes.

The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the arabidopsis thaliana protein MFP2.,Arent S, Christensen CE, Pye VE, Norgaard A, Henriksen A J Biol Chem. 2010 Jul 30;285(31):24066-77. Epub 2010 May 12. PMID:20463021^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Richmond TA, Bleecker AB. A defect in beta-oxidation causes abnormal inflorescence development in Arabidopsis. Plant Cell. 1999 Oct;11(10):1911-24. PMID:10521521
↑ Rylott EL, Eastmond PJ, Gilday AD, Slocombe SP, Larson TR, Baker A, Graham IA. The Arabidopsis thaliana multifunctional protein gene (MFP2) of peroxisomal beta-oxidation is essential for seedling establishment. Plant J. 2006 Mar;45(6):930-41. PMID:16507084 doi:10.1111/j.1365-313X.2005.02650.x
↑ Arent S, Christensen CE, Pye VE, Norgaard A, Henriksen A. The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the arabidopsis thaliana protein MFP2. J Biol Chem. 2010 Jul 30;285(31):24066-77. Epub 2010 May 12. PMID:20463021 doi:10.1074/jbc.M110.106005
↑ Arent S, Christensen CE, Pye VE, Norgaard A, Henriksen A. The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the arabidopsis thaliana protein MFP2. J Biol Chem. 2010 Jul 30;285(31):24066-77. Epub 2010 May 12. PMID:20463021 doi:10.1074/jbc.M110.106005

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wtb"

@@ Line 1: / Line 1: @@
 ==ARABIDOPSIS THALIANA MULTIFUCTIONAL PROTEIN, MFP2==
 <StructureSection load='2wtb' size='340' side='right' caption='[[2wtb]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wtb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WTB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wtb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WTB FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wtb RCSB], [http://www.ebi.ac.uk/pdbsum/2wtb PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wtb OCA], [http://pdbe.org/2wtb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wtb RCSB], [http://www.ebi.ac.uk/pdbsum/2wtb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wtb ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q9ZPI5_ARATH Q9ZPI5_ARATH]] Involved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18).<ref>PMID:10521521</ref> <ref>PMID:16507084</ref> <ref>PMID:20463021</ref>
+[[http://www.uniprot.org/uniprot/MFP2_ARATH MFP2_ARATH]] Involved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18).<ref>PMID:10521521</ref> <ref>PMID:16507084</ref> <ref>PMID:20463021</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 16: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wtb ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 25: / Line 26: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2wtb" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Arabidopsis thaliana]]
+[[Category: Arath]]
 [[Category: Arent, S]]
 [[Category: Christensen, C E]]

2wtb

From Proteopedia

Revision as of 11:41, 4 August 2016

ARABIDOPSIS THALIANA MULTIFUCTIONAL PROTEIN, MFP2

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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