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3w15

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Revision as of 11:54, 4 August 2016

Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p

Structural highlights

3w15 is a 3 chain structure with sequence from Baker's yeast and Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	PEX7 (Baker's yeast), PEX21 (Baker's yeast), FOX3 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PEX7_YEAST] Binds to the N-terminal PTS2-type peroxisomal targeting signal and plays an essential role in peroxisomal protein import essential for import of 3-oxoacyl-CoA thiolase (a PTS2-containing protein) into peroxisomes. May direct thiolase to peroxisomes by shuttling between the cytosol and peroxisomal membranes. [PEX21_YEAST] Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Acts as an activator of the seryl-tRNA synthetase SES1 by increasing its binding to tRNA.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Appropriate targeting of matrix proteins to peroxisomes is mainly directed by two types of peroxisomal targeting signals, PTS1 and PTS2. Although the basis of PTS1 recognition has been revealed by structural studies, that of PTS2 recognition remains elusive. Here we present the crystal structure of a heterotrimeric PTS2-recognition complex from Saccharomyces cerevisiae, containing Pex7p, the C-terminal region of Pex21p and the PTS2 of the peroxisomal 3-ketoacyl-CoA thiolase. Pex7p forms a beta-propeller structure and provides a platform for cooperative interactions with both the amphipathic PTS2 helix and Pex21p. The C-terminal region of Pex21p directly covers the hydrophobic surfaces of both Pex7p and PTS2, and the resulting hydrophobic core is the primary determinant of stable complex formation. Together with in vivo and in vitro functional assays of Pex7p and Pex21p variants, our findings reveal the molecular mechanism of PTS2 recognition.

Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p.,Pan D, Nakatsu T, Kato H Nat Struct Mol Biol. 2013 Aug;20(8):987-93. doi: 10.1038/nsmb.2618. Epub 2013 Jun, 30. PMID:23812376^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Purdue PE, Yang X, Lazarow PB. Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway. J Cell Biol. 1998 Dec 28;143(7):1859-69. PMID:9864360
↑ Rocak S, Landeka I, Weygand-Durasevic I. Identifying Pex21p as a protein that specifically interacts with yeast seryl-tRNA synthetase. FEMS Microbiol Lett. 2002 Aug 27;214(1):101-6. PMID:12204379
↑ Stein K, Schell-Steven A, Erdmann R, Rottensteiner H. Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol. 2002 Sep;22(17):6056-69. PMID:12167700
↑ Godinic V, Mocibob M, Rocak S, Ibba M, Weygand-Durasevic I. Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA(Ser). FEBS J. 2007 Jun;274(11):2788-99. Epub 2007 Apr 20. PMID:17451428 doi:10.1111/j.1742-4658.2007.05812.x
↑ Pan D, Nakatsu T, Kato H. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nat Struct Mol Biol. 2013 Aug;20(8):987-93. doi: 10.1038/nsmb.2618. Epub 2013 Jun, 30. PMID:23812376 doi:http://dx.doi.org/10.1038/nsmb.2618

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3w15"

@@ Line 1: / Line 1: @@
 ==Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p==
 <StructureSection load='3w15' size='340' side='right' caption='[[3w15]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3w15]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bionectria_wenpingii Bionectria wenpingii], [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W15 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3w15]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W15 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PEX7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=466134 Bionectria wenpingii]), PEX21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), FOX3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PEX7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PEX21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), FOX3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w15 RCSB], [http://www.ebi.ac.uk/pdbsum/3w15 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w15 OCA], [http://pdbe.org/3w15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w15 RCSB], [http://www.ebi.ac.uk/pdbsum/3w15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w15 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3w15" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bionectria wenpingii]]
+[[Category: Baker's yeast]]
 [[Category: Ecoli]]
-[[Category: Saccharomyces cerevisiae s288c]]
 [[Category: Kato, H]]
 [[Category: Nakatsu, T]]

3w15

From Proteopedia

Revision as of 11:54, 4 August 2016

Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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