1ktb
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ktb |SIZE=350|CAPTION= <scene name='initialview01'>1ktb</scene>, resolution 1.90Å | |PDB= 1ktb |SIZE=350|CAPTION= <scene name='initialview01'>1ktb</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-N-acetylgalactosaminidase Alpha-N-acetylgalactosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.49 3.2.1.49] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-N-acetylgalactosaminidase Alpha-N-acetylgalactosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.49 3.2.1.49] </span> |
|GENE= alpha-NAGA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | |GENE= alpha-NAGA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ktc|1KTC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ktb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ktb OCA], [http://www.ebi.ac.uk/pdbsum/1ktb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ktb RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Hannick, L.]] | [[Category: Hannick, L.]] | ||
[[Category: Zhu, A.]] | [[Category: Zhu, A.]] | ||
| - | [[Category: | + | [[Category: (beta/alpha)8 barrel]] |
| - | + | [[Category: glycoprotein]] | |
| - | + | ||
| - | + | ||
| - | [[Category: glycoprotein | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:53:18 2008'' |
Revision as of 18:53, 30 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | alpha-NAGA (Gallus gallus) | ||||||
| Activity: | Alpha-N-acetylgalactosaminidase, with EC number 3.2.1.49 | ||||||
| Related: | 1KTC
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure of alpha-N-Acetylgalactosaminidase
Overview
In the lysosome, glycosidases degrade glycolipids, glycoproteins, and oligosaccharides. Mutations in glycosidases cause disorders characterized by the deposition of undegraded carbohydrates. Schindler and Fabry diseases are caused by the incomplete degradation of carbohydrates with terminal alpha-N-acetylgalactosamine and alpha-galactose, respectively. Here we present the X-ray structure of alpha-N-acetylgalactosaminidase (alpha-NAGAL), the glycosidase that removes alpha-N-acetylgalactosamine, and the structure with bound ligand. The active site residues of alpha-NAGAL are conserved in the closely related enzyme a-galactosidase A (alpha-GAL). The structure demonstrates the catalytic mechanisms of both enzymes and reveals the structural basis of mutations causing Schindler and Fabry diseases. As alpha-NAGAL and alpha-GAL produce type O "universal donor" blood from type A and type B blood, the alpha-NAGAL structure will aid in the engineering of improved enzymes for blood conversion.
About this Structure
1KTB is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
The 1.9 A structure of alpha-N-acetylgalactosaminidase: molecular basis of glycosidase deficiency diseases., Garman SC, Hannick L, Zhu A, Garboczi DN, Structure. 2002 Mar;10(3):425-34. PMID:12005440
Page seeded by OCA on Sun Mar 30 21:53:18 2008
