1kuj
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ku8|1ku8]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kuj OCA], [http://www.ebi.ac.uk/pdbsum/1kuj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kuj RCSB]</span> | ||
}} | }} | ||
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[[Category: Rouge, P.]] | [[Category: Rouge, P.]] | ||
[[Category: Zamboni, V.]] | [[Category: Zamboni, V.]] | ||
| - | [[Category: MMA]] | ||
[[Category: beta-prism fold]] | [[Category: beta-prism fold]] | ||
[[Category: carbohydrate binding]] | [[Category: carbohydrate binding]] | ||
[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:53:41 2008'' |
Revision as of 18:53, 30 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1ku8
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose
Overview
Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
About this Structure
1KUJ is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.
Reference
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090
Page seeded by OCA on Sun Mar 30 21:53:41 2008
