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3v17

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Revision as of 12:51, 4 August 2016

Crystal structure of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440

Structural highlights

3v17 is a 4 chain structure with sequence from Psepk. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3pvj, 3v15
Gene:	PP0230, PP_0230, tauD (PSEPK)
Activity:	Taurine dioxygenase, with EC number 1.14.11.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Fe(II)/alpha-ketoglutarate dependent oxygenases are versatile catalysts associated with a number of different biological functions, in which they use the oxidizing power of activated dioxygen to convert a variety of substrates. A mononuclear non-heme iron center is used to couple the decarboxylation of the cosubstrate alpha-ketoglutarate with a two-electron oxidation of the substrate, which is a hydroxylation in most cases. Although Fe(II)/alpha-ketoglutarate dependent oxygenases have diverse amino acid sequences and substrate specifity, it is assumed that they share a common mechanism. One representative of this enzyme family is the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase (TauD) that catalyzes the hydroxylation of taurine yielding sulfite and aminoacetaldehyde. Its mechanism has been studied in detail becoming a model system for the whole enzyme family. However, its oligomeric state and architecture have been disputed. Here, we report the biochemical and kinetic characterization of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440 (TauD(Pp) ). We also present three crystal structures of the apo form of this enzyme. Comparisons with TauD from Escherichia coli (TauD(Ec) ) demonstrate that both enzymes are quite similar regarding their spectra, structure and kinetics and only minor differences for the accumulation of intermediates during the reaction have been observed. Structural data and the analytical gelfiltration as well as sedimentation velocity analytical ultracentrifugation show that both TauD(Pp) and TauD(Ec) are tetramers in solution and in the crystals, which is in contrast to the earlier description of TauD from E. coli as a dimer. Structured digital abstract tauDpp and tauDpp bind by molecularsieving (Viewinteraction) tauDpp and tauDpp bind by x-raycrystallography (Viewinteraction) tauDEc and tauDEc bind by molecularsieving (Viewinteraction).

The Fe(II) /alpha-ketoglutarate dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers.,Knauer SH, Hartl-Spiegelhauer O, Schwarzinger S, Hanzelmann P, Dobbek H FEBS J. 2012 Jan 5. doi: 10.1111/j.1742-4658.2012.08473.x. PMID:22221834^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Knauer SH, Hartl-Spiegelhauer O, Schwarzinger S, Hanzelmann P, Dobbek H. The Fe(II) /alpha-ketoglutarate dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers. FEBS J. 2012 Jan 5. doi: 10.1111/j.1742-4658.2012.08473.x. PMID:22221834 doi:10.1111/j.1742-4658.2012.08473.x

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3v17"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440==
 <StructureSection load='3v17' size='340' side='right' caption='[[3v17]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3v17]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida_kt2440 Pseudomonas putida kt2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V17 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3v17]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepk Psepk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V17 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pvj|3pvj]], [[3v15|3v15]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PP0230, PP_0230, tauD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 Pseudomonas putida KT2440])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PP0230, PP_0230, tauD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Taurine_dioxygenase Taurine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.17 1.14.11.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v17 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v17 RCSB], [http://www.ebi.ac.uk/pdbsum/3v17 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v17 OCA], [http://pdbe.org/3v17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v17 RCSB], [http://www.ebi.ac.uk/pdbsum/3v17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v17 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3v17" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Pseudomonas putida kt2440]]
+[[Category: Psepk]]
 [[Category: Taurine dioxygenase]]
 [[Category: Dobbek, H]]

3v17

From Proteopedia

Revision as of 12:51, 4 August 2016

Crystal structure of the Fe(II)/alpha-ketoglutarate dependent taurine dioxygenase from Pseudomonas putida KT2440

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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