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3pto

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Revision as of 14:58, 4 August 2016

Crystal Structure of an empty Vesicular Stomatitis Virus Nucleocapsid Protein Complex

Structural highlights

3pto is a 5 chain structure with sequence from Vsiv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3ptx, 3pu0, 3pu1, 3pu4
Gene:	N (VSIV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).

Publication Abstract from PubMed

The genomic RNA of negative strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in E. coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Poly-ribonucleotides could be re-encapsidated into the empty NLP and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.

Access of RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.,Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M J Virol. 2010 Dec 22. PMID:21177817^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M. Access of RNA encapsidated in the nucleocapsid of vesicular stomatitis virus. J Virol. 2010 Dec 22. PMID:21177817 doi:10.1128/JVI.01927-10

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3pto"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of an empty Vesicular Stomatitis Virus Nucleocapsid Protein Complex==
 <StructureSection load='3pto' size='340' side='right' caption='[[3pto]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3pto]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PTO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3pto]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PTO FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ptx|3ptx]], [[3pu0|3pu0]], [[3pu1|3pu1]], [[3pu4|3pu4]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pto OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pto RCSB], [http://www.ebi.ac.uk/pdbsum/3pto PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pto OCA], [http://pdbe.org/3pto PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pto RCSB], [http://www.ebi.ac.uk/pdbsum/3pto PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pto ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3pto" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 25: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Vesicular stomatitis indiana virus]]
+[[Category: Vsiv]]
 [[Category: Green, T J]]
 [[Category: Luo, M]]

3pto

From Proteopedia

Revision as of 14:58, 4 August 2016

Crystal Structure of an empty Vesicular Stomatitis Virus Nucleocapsid Protein Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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