This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3tl1

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 18:03, 4 August 2016

Crystal structure of the Streptomyces coelicolor WhiE ORFVI polyketide aromatase/cyclase

Structural highlights

3tl1 is a 2 chain structure with sequence from "actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	SCO5315, SC6G9.18 ("Actinomyces coelicolor" (Muller 1908) Lieske 1921)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CYPC_STRCO] Involved in developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics which is essential for spore color in Streptomyces coelicolor.

Publication Abstract from PubMed

Aromatic polyketides are biologically active natural products. Many important pharmaceuticals are derived from aromatic polyketides. Especially important in aromatic polyketide biosynthesis is the regiospecific cyclization of a linear, preassembled polyketide chain catalyzed by aromatase/cyclase (ARO/CYC), which serves as a key control point in aromatic ring formation. How different ARO/CYCs promote different cyclization patterns is not well understood. The whiE locus of Streptomyces coelicolor A3(2) is responsible for the biosynthesis of an aromatic polyketide precursor to the gray spore pigment. The WhiE ARO/CYC catalyzes the regiospecific C9-C14 and C7-C16 cyclization and aromatization of a 24-carbon polyketide chain. WhiE ARO/CYC shares a high degree of similarity to another nonreducing PKS ARO/CYC, TcmN ARO/CYC. This paper presents the apo crystal structure of WhiE ARO/CYC, and cocrystal structures of WhiE and TcmN ARO/CYCs bound with polycyclic aromatic compounds that mimic the respective ARO/CYC products. Site-directed mutagenesis coupled with in vitro PKS reconstitution assays was used to characterize the interior pocket residues of WhiE ARO/CYC. The results confirmed that the interior pocket of ARO/CYCs is a critical determinant of polyketide cyclization specificity. A unified ARO/CYC-mediated cyclization mechanism is proposed on the basis of these structural and functional results.

Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI.,Lee MY, Ames BD, Tsai SC Biochemistry. 2012 Mar 30. PMID:22432862^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee MY, Ames BD, Tsai SC. Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI. Biochemistry. 2012 Mar 30. PMID:22432862 doi:10.1021/bi201705q

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tl1"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Streptomyces coelicolor WhiE ORFVI polyketide aromatase/cyclase==
 <StructureSection load='3tl1' size='340' side='right' caption='[[3tl1]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=JRO:6,7,9-TRIHYDROXY-3-METHYL-1H-BENZO[G]ISOCHROMEN-1-ONE'>JRO</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCO5315, SC6G9.18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tl1 RCSB], [http://www.ebi.ac.uk/pdbsum/3tl1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl1 OCA], [http://pdbe.org/3tl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tl1 RCSB], [http://www.ebi.ac.uk/pdbsum/3tl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tl1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3tl1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3tl1

From Proteopedia

Revision as of 18:03, 4 August 2016

Crystal structure of the Streptomyces coelicolor WhiE ORFVI polyketide aromatase/cyclase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox