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4k06
From Proteopedia
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| - | {{STRUCTURE_4k06| PDB=4k06 | SCENE= }} | ||
| - | ===Crystal structure of MTX-II from Bothrops brazili venom complexed with polyethylene glycol=== | ||
| - | {{ABSTRACT_PUBMED_24145104}} | ||
| - | == | + | ==Crystal structure of MTX-II from Bothrops brazili venom complexed with polyethylene glycol== |
| - | [[4k06]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_brazili Bothrops brazili]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K06 OCA]. | + | <StructureSection load='4k06' size='340' side='right' caption='[[4k06]], [[Resolution|resolution]] 2.08Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4k06]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_brazili Bothrops brazili]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K06 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K06 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7PE:2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL'>7PE</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iq3|3iq3]], [[3cyl|3cyl]], [[2ok9|2ok9]], [[4k09|4k09]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k06 OCA], [http://pdbe.org/4k06 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k06 RCSB], [http://www.ebi.ac.uk/pdbsum/4k06 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k06 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bothrops brazili is a snake found in the forests of the Amazonian region whose commercial therapeutic anti-bothropic serum has low efficacy for local myotoxic effects, resulting in an important public health problem in this area. Catalytically inactive phospholipases A2-like (Lys49-PLA2s) are among the main components from Bothrops genus venoms and are capable of causing drastic myonecrosis. Several studies have shown that the C-terminal region of these toxins, which includes a variable combination of positively charged and hydrophobic residues, is responsible for their activity. In this work we describe the crystal structures of two Lys49-PLA2s (BbTX-II and MTX-II) from B. brazili venom and a comprehensive structural comparison with several Lys49-PLA2s. Based on these results, two independent sites of interaction were identified between protein and membrane which leads to the proposition of a new myotoxic mechanism for bothropic Lys49-PLA2s composed of five different steps. This proposition is able to fully explain the action of these toxins and may be useful to develop efficient inhibitors to complement the conventional antivenom administration. | ||
| - | + | Structural bases for a complete myotoxic mechanism: Crystal structures of two non-catalytic phospholipases A-like from Bothrops brazili venom.,Fernandes CA, Comparetti EJ, Borges RJ, Huancahuire-Vega S, Ponce-Soto LA, Marangoni S, Soares AM, Fontes MR Biochim Biophys Acta. 2013 Oct 18;1834(12):2772-2781. doi:, 10.1016/j.bbapap.2013.10.009. PMID:24145104<ref>PMID:24145104</ref> | |
| - | <ref | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4k06" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bothrops brazili]] | [[Category: Bothrops brazili]] | ||
| - | [[Category: Borges, R J | + | [[Category: Borges, R J]] |
| - | [[Category: Comparetti, E J | + | [[Category: Comparetti, E J]] |
| - | [[Category: Fernandes, C A.H | + | [[Category: Fernandes, C A.H]] |
| - | [[Category: Fontes, M R.M | + | [[Category: Fontes, M R.M]] |
[[Category: Phospholipase a2]] | [[Category: Phospholipase a2]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 18:08, 4 August 2016
Crystal structure of MTX-II from Bothrops brazili venom complexed with polyethylene glycol
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