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4kf3

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Revision as of 18:36, 4 August 2016

Crystal Structure of Myotoxin II (MjTX-II), a myotoxic Lys49-phospholipase A2 from Bothrops moojeni.

Structural highlights

4kf3 is a 2 chain structure with sequence from Bothrops moojeni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1xxs, 3iq3, 3t0r, 2q2j
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PA2H2_BOTMO] Snake venom phospholipase A2 homolog that lack enzymatic activity. Displays myotoxin and edema-inducing activities.

Publication Abstract from PubMed

Lys49-phospholipases A2 (Lys49-PLA2s) are proteins found in bothropic snake venoms (Viperidae family) and belong to a class of proteins which presents a phospholipase A2 scaffold but are catalytically inactive. These proteins (also known as PLA2s-like toxins) exert a pronounced local myotoxic effect and are not neutralized by antivenom, being their study relevant in terms of medical and scientific interest. Despite of the several studies reported in the literature for this class of proteins only a partial consensus has been achieved concerning their functional-structural relationships. In this work, we present a comprehensive structural and functional study with the MjTX-II, a dimeric Lys49-PLA2 from Bothrops moojeni venom which includes: (i) high-resolution crystal structure; (ii) dynamic light scattering and bioinformatics studies in order to confirm its biological assembly; (iii) myographic and electrophysiological studies and, (iv) comparative studies with other Lys49-PLA2s. These comparative analyses let us to get important insights into the role of Lys122 amino acid, previously indicated as responsible for Lys49-PLA2s catalytic inactivity and added important elements to establish the correct biological assembly for this class of proteins. Furthermore, we show two unique sequential features of MjTX-II (an amino acid insertion and a mutation) in comparison to all bothropic Lys49-PLA2s that lead to a distinct way of ligand binding at the toxin's hydrophobic channel and also, allowed the presence of an additional ligand molecule in this region. These facts suggest a possible particular mode of binding for long-chain ligands that interacts with MjTX-II hydrophobic channel, a feature that may directly affect the design of structure-based ligands for Lys49-PLA2s.

Structural and functional studies with mytoxin II from Bothrops moojeni reveal remarkable similarities and differences compared to other catalytically inactive phospholipases A-like.,Salvador GH, Cavalcante WL, Dos Santos JI, Gallacci M, Soares AM, Fontes MR Toxicon. 2013 Jun 25;72C:52-63. doi: 10.1016/j.toxicon.2013.06.013. PMID:23810946^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Salvador GH, Cavalcante WL, Dos Santos JI, Gallacci M, Soares AM, Fontes MR. Structural and functional studies with mytoxin II from Bothrops moojeni reveal remarkable similarities and differences compared to other catalytically inactive phospholipases A-like. Toxicon. 2013 Jun 25;72C:52-63. doi: 10.1016/j.toxicon.2013.06.013. PMID:23810946 doi:10.1016/j.toxicon.2013.06.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kf3"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Myotoxin II (MjTX-II), a myotoxic Lys49-phospholipase A2 from Bothrops moojeni.==
 <StructureSection load='4kf3' size='340' side='right' caption='[[4kf3]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xxs|1xxs]], [[3iq3|3iq3]], [[3t0r|3t0r]], [[2q2j|2q2j]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kf3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kf3 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kf3 OCA], [http://pdbe.org/4kf3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kf3 RCSB], [http://www.ebi.ac.uk/pdbsum/4kf3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kf3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4kf3" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4kf3

From Proteopedia

Revision as of 18:36, 4 August 2016

Crystal Structure of Myotoxin II (MjTX-II), a myotoxic Lys49-phospholipase A2 from Bothrops moojeni.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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