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4tvw

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Revision as of 19:11, 4 August 2016

Resorufin ligase with bound resorufin-AMP analog

Structural highlights

4tvw is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3a7r, 4tvy
Activity:	Transferase, with EC number 2.7.7.63
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LPLA_ECOLI] Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Is also able to catalyze very poorly the transfer of lipoyl and octanoyl moiety from their acyl carrier protein.^[1]

Publication Abstract from PubMed

Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a fluorophore ligase that accepts the red dye resorufin, starting from Escherichia coli lipoic acid ligase. X-ray crystallography showed that the design closely matched the experimental structure. Resorufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular proteins genetically fused to a 13-aa recognition peptide in multiple mammalian cell lines and in primary cultured neurons. We used resorufin ligase to perform superresolution imaging of the intermediate filament protein vimentin by stimulated emission depletion and electron microscopies. This work illustrates the power of Rosetta for major redesign of enzyme specificity and introduces a tool for minimally invasive, highly specific imaging of cellular proteins by both conventional and superresolution microscopies.

Computational design of a red fluorophore ligase for site-specific protein labeling in living cells.,Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, Ting AY Proc Natl Acad Sci U S A. 2014 Oct 13. pii: 201404736. PMID:25313043^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Green DE, Morris TW, Green J, Cronan JE Jr, Guest JR. Purification and properties of the lipoate protein ligase of Escherichia coli. Biochem J. 1995 Aug 1;309 ( Pt 3):853-62. PMID:7639702
↑ Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, Ting AY. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells. Proc Natl Acad Sci U S A. 2014 Oct 13. pii: 201404736. PMID:25313043 doi:http://dx.doi.org/10.1073/pnas.1404736111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tvw"

Categories: Transferase | Drennan, C L | Goldman, P J | Computational enzyme design | E. coli lpla

@@ Line 1: / Line 1: @@
 ==Resorufin ligase with bound resorufin-AMP analog==
 <StructureSection load='4tvw' size='340' side='right' caption='[[4tvw]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a7r|3a7r]], [[4tvy|4tvy]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.63 2.7.7.63] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tvw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tvw RCSB], [http://www.ebi.ac.uk/pdbsum/4tvw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tvw OCA], [http://pdbe.org/4tvw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tvw RCSB], [http://www.ebi.ac.uk/pdbsum/4tvw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tvw ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4tvw" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4tvw

From Proteopedia

Revision as of 19:11, 4 August 2016

Resorufin ligase with bound resorufin-AMP analog

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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