1lgt
From Proteopedia
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|PDB= 1lgt |SIZE=350|CAPTION= <scene name='initialview01'>1lgt</scene>, resolution 1.70Å | |PDB= 1lgt |SIZE=350|CAPTION= <scene name='initialview01'>1lgt</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=BP3:2'-CHLORO-BIPHENYL-2,3-DIOL'>BP3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Biphenyl-2,3-diol_1,2-dioxygenase Biphenyl-2,3-diol 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.39 1.13.11.39] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Biphenyl-2,3-diol_1,2-dioxygenase Biphenyl-2,3-diol 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.39 1.13.11.39] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1han|1HAN]], [[1lkd|1LKD]], [[1kmy|1KMY]], [[1knd|1KND]], [[1knf|1KNF]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lgt OCA], [http://www.ebi.ac.uk/pdbsum/1lgt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lgt RCSB]</span> | ||
}} | }} | ||
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[[Category: Bolin, J T.]] | [[Category: Bolin, J T.]] | ||
[[Category: Dai, S.]] | [[Category: Dai, S.]] | ||
| - | + | [[Category: 2,3-dihydroxybiphenyl]] | |
| - | + | ||
| - | [[Category: 2 | + | |
| - | + | ||
[[Category: anaerobic]] | [[Category: anaerobic]] | ||
[[Category: extradiol dioxygenase]] | [[Category: extradiol dioxygenase]] | ||
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[[Category: pcb biodegradation]] | [[Category: pcb biodegradation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:02:41 2008'' |
Revision as of 19:02, 30 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Biphenyl-2,3-diol 1,2-dioxygenase, with EC number 1.13.11.39 | ||||||
| Related: | 1HAN, 1LKD, 1KMY, 1KND, 1KNF
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)
Overview
The microbial degradation of polychlorinated biphenyls (PCBs) provides the potential to destroy these widespread, toxic and persistent environmental pollutants. For example, the four-step upper bph pathway transforms some of the more than 100 different PCBs found in commercial mixtures and is being engineered for more effective PCB degradation. In the critical third step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD; EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its suicide inactivation and interfere with the degradation of other compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a factor of approximately 7,000 relative to DHB, and it bound with sufficient affinity to competitively inhibit DHB cleavage at nanomolar concentrations. Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies.
About this Structure
1LGT is a Single protein structure of sequence from Burkholderia sp.. Full crystallographic information is available from OCA.
Reference
Identification and analysis of a bottleneck in PCB biodegradation., Dai S, Vaillancourt FH, Maaroufi H, Drouin NM, Neau DB, Snieckus V, Bolin JT, Eltis LD, Nat Struct Biol. 2002 Dec;9(12):934-9. PMID:12415290
Page seeded by OCA on Sun Mar 30 22:02:41 2008
