1lj2
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1lj2 |SIZE=350|CAPTION= <scene name='initialview01'>1lj2</scene>, resolution 2.38Å | |PDB= 1lj2 |SIZE=350|CAPTION= <scene name='initialview01'>1lj2</scene>, resolution 2.38Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=AU:GOLD ION'>AU</scene> | + | |LIGAND= <scene name='pdbligand=AU:GOLD+ION'>AU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lj2 OCA], [http://www.ebi.ac.uk/pdbsum/1lj2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lj2 RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Burley, S K.]] | [[Category: Burley, S K.]] | ||
[[Category: Groft, C M.]] | [[Category: Groft, C M.]] | ||
| - | [[Category: | + | [[Category: closed loop]] |
| - | [[Category: | + | [[Category: coiled coil]] |
| + | [[Category: eif4g]] | ||
| + | [[Category: homodimer]] | ||
| + | [[Category: mrna]] | ||
| + | [[Category: nsp3]] | ||
| + | [[Category: rotavirus]] | ||
| + | [[Category: translation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:23 2008'' |
Revision as of 19:03, 30 March 2008
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| , resolution 2.38Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization
Overview
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
About this Structure
1LJ2 is a Protein complex structure of sequences from Simian rotavirus a/sa11. Full crystallographic information is available from OCA.
Reference
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization., Groft CM, Burley SK, Mol Cell. 2002 Jun;9(6):1273-83. PMID:12086624
Page seeded by OCA on Sun Mar 30 22:03:23 2008
Categories: Protein complex | Simian rotavirus a/sa11 | Burley, S K. | Groft, C M. | Closed loop | Coiled coil | Eif4g | Homodimer | Mrna | Nsp3 | Rotavirus | Translation
