1llf
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1llf |SIZE=350|CAPTION= <scene name='initialview01'>1llf</scene>, resolution 1.4Å | |PDB= 1llf |SIZE=350|CAPTION= <scene name='initialview01'>1llf</scene>, resolution 1.4Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=F23:TRICOSANOIC ACID'>F23</scene> | + | |LIGAND= <scene name='pdbligand=F23:TRICOSANOIC+ACID'>F23</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1cle|1CLE]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1llf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1llf OCA], [http://www.ebi.ac.uk/pdbsum/1llf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1llf RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Pletnev, V.]] | [[Category: Pletnev, V.]] | ||
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
| - | [[Category: F23]] | ||
[[Category: candida cylindracea cholesterol esterase]] | [[Category: candida cylindracea cholesterol esterase]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: sterol ester acylhydrolase]] | [[Category: sterol ester acylhydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:16 2008'' |
Revision as of 19:04, 30 March 2008
| |||||||
| , resolution 1.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||
| Related: | 1CLE
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution
Overview
The three-dimensional structure of a Candida cylindracea cholesterol esterase (ChE) homodimer (534 x 2 amino acids) in complex with a ligand of proposed formula C(23)H(48)O(2) has been determined at 1.4 A resolution in space group P1 using synchrotron low-temperature data. The structure refined to R = 0.136 and R(free) = 0.169 and has revealed new stereochemical details in addition to those detected for the apo- and holo-forms at 1.9 and 2.0 A resolution, respectively [Ghosh et al. (1995), Structure, 3, 279-288]. The cholesterol esterase structure is a dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity. Hydrophobic active-site gorges in each subunit face each other across a central interfacial cavity. The ChE subunits have carbohydrate chains attached to their Asn314 and Asn351 residues, with two ordered N-acetyl-D-glucosoamine moieties visible at each site. The side chains of 14 residues have two alternative conformations with occupancy values of 0.5 +/- 0.2. For each subunit the electron density in the enzyme active-site gorge is well modeled by a C(23)-chain fatty acid.
About this Structure
1LLF is a Single protein structure of sequence from Candida cylindracea. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution., Pletnev V, Addlagatta A, Wawrzak Z, Duax W, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):50-6. Epub 2002 Dec, 19. PMID:12499539
Page seeded by OCA on Sun Mar 30 22:04:16 2008
