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4p3a

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Revision as of 00:54, 5 August 2016

Crystal structure of the mouse C5a anaphylatoxin

Structural highlights

4p3a is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4p39, 4p3b
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CO5_MOUSE] Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).

Publication Abstract from PubMed

Complement is an ancient part of the innate immune system that plays a pivotal role in protection against invading pathogens and helps to clear apoptotic and necrotic cells. Upon complement activation, a cascade of proteolytic events generates the complement effectors, including the anaphylatoxins C3a and C5a. Signalling through their cognate G-protein coupled receptors, C3aR and C5aR, leads to a wide range of biological events promoting inflammation at the site of complement activation. The function of anaphylatoxins is regulated by circulating carboxypeptidases that remove their C-terminal arginine residue, yielding C3a-desArg and C5a-desArg. Whereas human C3a and C3a-desArg adopt a canonical four-helix bundle fold, the conformation of human C5a-desArg has recently been described as a three-helix bundle. Here, the crystal structures of an antagonist version of human C5a, A8(Delta71-73), and of murine C5a and C5a-desArg are reported. Whereas A8(Delta71-73) adopts a three-helix bundle conformation similar to human C5a-desArg, the two murine proteins form a four-helix bundle. A cell-based functional assay reveals that murine C5a-desArg, in contrast to its human counterpart, exerts the same level of activition as murine C5a on its cognate receptor. The role of the different C5a conformations is discussed in relation to the differential activation of C5a receptors across species.

Structural and functional characterization of human and murine C5a anaphylatoxins.,Schatz-Jakobsen JA, Yatime L, Larsen C, Petersen SV, Klos A, Andersen GR Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1704-17. doi:, 10.1107/S139900471400844X. Epub 2014 May 30. PMID:24914981^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schatz-Jakobsen JA, Yatime L, Larsen C, Petersen SV, Klos A, Andersen GR. Structural and functional characterization of human and murine C5a anaphylatoxins. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1704-17. doi:, 10.1107/S139900471400844X. Epub 2014 May 30. PMID:24914981 doi:http://dx.doi.org/10.1107/S139900471400844X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4p3a"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the mouse C5a anaphylatoxin==
 <StructureSection load='4p3a' size='340' side='right' caption='[[4p3a]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p39|4p39]], [[4p3b|4p3b]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p3a RCSB], [http://www.ebi.ac.uk/pdbsum/4p3a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p3a OCA], [http://pdbe.org/4p3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p3a RCSB], [http://www.ebi.ac.uk/pdbsum/4p3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p3a ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4p3a" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Complement C5|Complement C5]]
 == References ==
 <references/>

4p3a

From Proteopedia

Revision as of 00:54, 5 August 2016

Crystal structure of the mouse C5a anaphylatoxin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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