4lfv

From Proteopedia

(Difference between revisions)

Revision as of 02:37, 5 August 2016

Crystal structure of human FPPS in complex with YS0470 and two molecules of inorganic phosphate

Structural highlights

4lfv is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	4dem, 4h5c, 4h5d, 4h5e, 4lfw
Gene:	FDPS, FPS, KIAA1293 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FPPS_HUMAN] Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Publication Abstract from PubMed

Human farnesyl pyrophosphate synthase (hFPPS) produces farnesyl pyrophosphate, an isoprenoid essential for a variety of cellular processes. The enzyme has been well established as the molecular target of the nitrogen-containing bisphosphonates (N-BPs), which are best known for their antiresorptive effects in bone but are also known for their anticancer properties. Crystal structures of hFPPS in ternary complexes with a novel bisphosphonate, YS0470, and the secondary ligands inorganic phosphate (Pi), inorganic pyrophosphate (PPi) and isopentenyl pyrophosphate (IPP) have recently been reported. Only the co-binding of the bisphosphonate with either PPi or IPP resulted in the full closure of the C-terminal tail of the enzyme, a conformational change that is required for catalysis and that is also responsible for the potent in vivo efficacy of N-BPs. In the present communication, a co-crystal structure of hFPPS in complex with YS0470 and two molecules of Pi is reported. The unusually close proximity between these ligands, which was confirmed by anomalous diffraction data, suggests that they interact with one another, with their anionic charges neutralized in their bound state. The structure also showed the tail of the enzyme to be fully disordered, indicating that simultaneous binding of two Pi molecules with a bisphosphonate cannot induce the tail-closing conformational change in hFPPS. Examination of homologous FPPSs suggested that this ligand-dependent tail closure is only conserved in the mammalian proteins. The prevalence of Pi-bound hFPPS structures in the PDB raises a question regarding the in vivo relevance of Pi binding to the function of the enzyme.

Structure of human farnesyl pyrophosphate synthase in complex with an aminopyridine bisphosphonate and two molecules of inorganic phosphate.,Park J, Lin YS, Tsantrizos YS, Berghuis AM Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):299-304. doi:, 10.1107/S2053230X14002106. Epub 2014 Feb 19. PMID:24598914^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Park J, Lin YS, Tsantrizos YS, Berghuis AM. Structure of human farnesyl pyrophosphate synthase in complex with an aminopyridine bisphosphonate and two molecules of inorganic phosphate. Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):299-304. doi:, 10.1107/S2053230X14002106. Epub 2014 Feb 19. PMID:24598914 doi:http://dx.doi.org/10.1107/S2053230X14002106

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lfv"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human FPPS in complex with YS0470 and two molecules of inorganic phosphate==
 <StructureSection load='4lfv' size='340' side='right' caption='[[4lfv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dem|4dem]], [[4h5c|4h5c]], [[4h5d|4h5d]], [[4h5e|4h5e]], [[4lfw|4lfw]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FDPS, FPS, KIAA1293 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lfv RCSB], [http://www.ebi.ac.uk/pdbsum/4lfv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lfv OCA], [http://pdbe.org/4lfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lfv RCSB], [http://www.ebi.ac.uk/pdbsum/4lfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lfv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4lfv" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Farnesyl diphosphate synthase|Farnesyl diphosphate synthase]]
 == References ==
 <references/>

4lfv

From Proteopedia

Revision as of 02:37, 5 August 2016

Crystal structure of human FPPS in complex with YS0470 and two molecules of inorganic phosphate

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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