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4nzw

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Revision as of 02:44, 5 August 2016

Crystal Structure of STK25-MO25 Complex

Structural highlights

4nzw is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAB39_HUMAN] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [STK25_HUMAN] Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.^[1]

Publication Abstract from PubMed

The tumor suppressor kinase LKB1 and germinal center kinases (GCKs) are key regulators of various cellular functions. The adaptor molecule MO25 not only recruits and activates LKB1 through the pseudokinase STRAD, but also may directly activate GCKs like MST3, MST4, STK25, OSR1 and SPAK. Targeting MO25 in a pathological setting has been recently studied in mouse. Yet the regulatory mechanism of MO25-mediated kinase activation is not fully understood. Here, our structural studies of MO25-related kinases reveal that MO25 binds to and activates GCK kinases or pseudokinase through a unified structural mechanism, featuring an active conformation of the alphaC helix and A-loop stabilized by MO25. Compared to GCKs that are directly activated by MO25-binding, activation of LKB1 has evolved additional layer of regulatory machinery, i.e., MO25 "activates" the pseudokinase STRAD, which in turn activates LKB1. Importantly, the structures of MO25alpha-STK25 and MO25alpha-MST3 determined in this work represent a transition/intermediate state and a fully activated state, respectively during the MO25-mediated kinase activating process.

Structural insights into regulatory mechanisms of MO25-mediated kinase activation.,Hao Q, Feng M, Shi Z, Li C, Chen M, Wang W, Zhang M, Jiao S, Zhou Z J Struct Biol. 2014 May;186(2):224-33. doi: 10.1016/j.jsb.2014.04.005. Epub 2014 , Apr 16. PMID:24746913^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Preisinger C, Short B, De Corte V, Bruyneel E, Haas A, Kopajtich R, Gettemans J, Barr FA. YSK1 is activated by the Golgi matrix protein GM130 and plays a role in cell migration through its substrate 14-3-3zeta. J Cell Biol. 2004 Mar 29;164(7):1009-20. Epub 2004 Mar 22. PMID:15037601 doi:10.1083/jcb.200310061
↑ Hao Q, Feng M, Shi Z, Li C, Chen M, Wang W, Zhang M, Jiao S, Zhou Z. Structural insights into regulatory mechanisms of MO25-mediated kinase activation. J Struct Biol. 2014 May;186(2):224-33. doi: 10.1016/j.jsb.2014.04.005. Epub 2014 , Apr 16. PMID:24746913 doi:http://dx.doi.org/10.1016/j.jsb.2014.04.005

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4nzw"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of STK25-MO25 Complex==
 <StructureSection load='4nzw' size='340' side='right' caption='[[4nzw]], [[Resolution|resolution]] 3.58&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=J60:5-[(E)-(5-CHLORO-2-OXO-1,2-DIHYDRO-3H-INDOL-3-YLIDENE)METHYL]-N-[2-(DIETHYLAMINO)ETHYL]-2,4-DIMETHYL-1H-PYRROLE-3-CARBOXAMIDE'>J60</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nzw RCSB], [http://www.ebi.ac.uk/pdbsum/4nzw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nzw OCA], [http://pdbe.org/4nzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nzw RCSB], [http://www.ebi.ac.uk/pdbsum/4nzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nzw ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4nzw" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Serine/threonine protein kinase|Serine/threonine protein kinase]]
 == References ==
 <references/>

4nzw

From Proteopedia

Revision as of 02:44, 5 August 2016

Crystal Structure of STK25-MO25 Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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