1lte
From Proteopedia
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|PDB= 1lte |SIZE=350|CAPTION= <scene name='initialview01'>1lte</scene>, resolution 2.0Å | |PDB= 1lte |SIZE=350|CAPTION= <scene name='initialview01'>1lte</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lte OCA], [http://www.ebi.ac.uk/pdbsum/1lte PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lte RCSB]</span> | ||
}} | }} | ||
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[[Category: Shaanan, B.]] | [[Category: Shaanan, B.]] | ||
[[Category: Sharon, N.]] | [[Category: Sharon, N.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MN]] | ||
[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:09 2008'' |
Revision as of 19:07, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF A LEGUME LECTIN WITH AN ORDERED N-LINKED CARBOHYDRATE IN COMPLEX WITH LACTOSE
Overview
The three-dimensional structure of the lactose complex of the Erythrina corallodendron lectin (EcorL), a dimer of N-glycosylated subunits, was determined crystallographically and refined at 2.0 angstrom resolution to an R value of 0.19. The tertiary structure of the subunit is similar to that of other legume lectins, but interference by the bulky N-linked heptasaccharide, which is exceptionally well ordered in the crystal, forces the EcorL dimer into a drastically different quaternary structure. Only the galactose moiety of the lactose ligand resides within the combining site. The galactose moiety is oriented differently from ligands in the mannose-glucose specific legume lectins and is held by hydrophobic interactions with Ala88, Tyr106, Phe131, and Ala218 and by seven hydrogen bonds, four of which are to the conserved Asp89, Asn133, and NH of Gly107. The specificity of legume lectins toward the different C-4 epimers appears to be associated with extensive variations in the outline of the variable parts of the binding sites.
About this Structure
1LTE is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a legume lectin with an ordered N-linked carbohydrate in complex with lactose., Shaanan B, Lis H, Sharon N, Science. 1991 Nov 8;254(5033):862-6. PMID:1948067
Page seeded by OCA on Sun Mar 30 22:07:09 2008
