1lua
From Proteopedia
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|PDB= 1lua |SIZE=350|CAPTION= <scene name='initialview01'>1lua</scene>, resolution 1.90Å | |PDB= 1lua |SIZE=350|CAPTION= <scene name='initialview01'>1lua</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NAP:NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NAP</scene> | + | |LIGAND= <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_dehydrogenase_(NADP(+)) Methylenetetrahydrofolate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.5 1.5.1.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_dehydrogenase_(NADP(+)) Methylenetetrahydrofolate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.5 1.5.1.5] </span> |
|GENE= mtdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=408 Methylobacterium extorquens]) | |GENE= mtdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=408 Methylobacterium extorquens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1lu9|1LU9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lua OCA], [http://www.ebi.ac.uk/pdbsum/1lua PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lua RCSB]</span> | ||
}} | }} | ||
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[[Category: Vorholt, J A.]] | [[Category: Vorholt, J A.]] | ||
[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
| - | [[Category: NAP]] | ||
[[Category: alpha/beta twisted open sheet structure]] | [[Category: alpha/beta twisted open sheet structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:26 2008'' |
Revision as of 19:07, 30 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | mtdA (Methylobacterium extorquens) | ||||||
| Activity: | Methylenetetrahydrofolate dehydrogenase (NADP(+)), with EC number 1.5.1.5 | ||||||
| Related: | 1LU9
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP
Overview
NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.
About this Structure
1LUA is a Single protein structure of sequence from Methylobacterium extorquens. Full crystallographic information is available from OCA.
Reference
Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:12176390
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