1lv2
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1lv2 |SIZE=350|CAPTION= <scene name='initialview01'>1lv2</scene>, resolution 2.7Å | |PDB= 1lv2 |SIZE=350|CAPTION= <scene name='initialview01'>1lv2</scene>, resolution 2.7Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PLM:PALMITIC ACID'>PLM</scene> | + | |LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= HNF4G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= HNF4G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv2 OCA], [http://www.ebi.ac.uk/pdbsum/1lv2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lv2 RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: Willson, T M.]] | [[Category: Willson, T M.]] | ||
[[Category: Wisely, B.]] | [[Category: Wisely, B.]] | ||
| - | [[Category: PLM]] | ||
[[Category: diabetes]] | [[Category: diabetes]] | ||
[[Category: fatty acid]] | [[Category: fatty acid]] | ||
| Line 40: | Line 42: | ||
[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:44 2008'' |
Revision as of 19:07, 30 March 2008
| |||||||
| , resolution 2.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | HNF4G (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids
Overview
The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain (LBD) revealed the presence of a fatty acid within the pocket, with the AF2 helix in a conformation characteristic of a transcriptionally active nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts from purified HNF4alpha and HNF4gamma LBDs identified mixtures of saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4 LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes show high constitutive activity in transient transfection assays, which was reduced by mutations designed to interfere with fatty acid binding. The endogenous fatty acids did not readily exchange with radiolabeled palmitic acid, and all attempts to displace them without denaturing the protein failed. Our results suggest that the HNF4s may be transcription factors that are constitutively bound to fatty acids.
About this Structure
1LV2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Hepatocyte nuclear factor 4 is a transcription factor that constitutively binds fatty acids., Wisely GB, Miller AB, Davis RG, Thornquest AD Jr, Johnson R, Spitzer T, Sefler A, Shearer B, Moore JT, Miller AB, Willson TM, Williams SP, Structure. 2002 Sep;10(9):1225-34. PMID:12220494
Page seeded by OCA on Sun Mar 30 22:07:44 2008
Categories: Homo sapiens | Single protein | Davis, R G. | Johnson, R. | Miller, A B. | Moore, J T. | Sefler, A. | Shearer, B. | Spitzer, T. | Williams, S P. | Willson, T M. | Wisely, B. | Diabetes | Fatty acid | Hnf4 | Mody | Nuclear receptor | Transcription factor
