This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lxh
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1lxh |SIZE=350|CAPTION= <scene name='initialview01'>1lxh</scene> | |PDB= 1lxh |SIZE=350|CAPTION= <scene name='initialview01'>1lxh</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1lxg|1lxg]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lxh OCA], [http://www.ebi.ac.uk/pdbsum/1lxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lxh RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Hawrot, E.]] | [[Category: Hawrot, E.]] | ||
[[Category: Zeng, H.]] | [[Category: Zeng, H.]] | ||
| - | [[Category: alpha-cobratoxin]] | ||
[[Category: nicotinic acetylcholine receptor]] | [[Category: nicotinic acetylcholine receptor]] | ||
[[Category: protein-protein interaction]] | [[Category: protein-protein interaction]] | ||
| - | [[Category: toxin]] | + | [[Category: toxin,alpha-cobratoxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:08:36 2008'' |
Revision as of 19:08, 30 March 2008
| |||||||
| Ligands: | |||||||
| Related: | 1lxg
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of alpha-cobratoxin complexed with a cognate peptide (minimized average structure)
Overview
The alpha18-mer peptide, spanning residues 181-198 of the Torpedo nicotinic acetylcholine receptor alpha1 subunit, contains key binding determinants for agonists and competitive antagonists. To investigate whether the alpha18-mer can bind other alpha-neurotoxins besides alpha-bungarotoxin, we designed a two-dimensional (1)H-(15)N heteronuclear single quantum correlation experiment to screen four related neurotoxins for their binding ability to the peptide. Of the four toxins tested (erabutoxin a, erabutoxin b, LSIII, and alpha-cobratoxin), only alpha-cobratoxin binds the alpha18-mer to form a 1:1 complex. The NMR solution structure of the alpha-cobratoxin.alpha18-mer complex was determined with a backbone root mean square deviation of 1.46 A. In the structure, alpha-cobratoxin contacts the alpha18-mer at the tips of loop I and II and through C-terminal cationic residues. The contact zone derived from the intermolecular nuclear Overhauser effects is in agreement with recent biochemical data. Furthermore, the structural models support the involvement of cation-pi interactions in stabilizing the complex. In addition, the binding screen results suggest that C-terminal cationic residues of alpha-bungarotoxin and alpha-cobratoxin contribute significantly to binding of the alpha18-mer. Finally, we present a structural model for nicotinic acetylcholine receptor-alpha-cobratoxin interaction by superimposing the alpha-cobratoxin.alpha18-mer complex onto the crystal structure of the acetylcholine-binding protein (Protein Data Bank code ).
About this Structure
1LXH is a Protein complex structure of sequences from Naja kaouthia and Torpedo californica. Full crystallographic information is available from OCA.
Reference
NMR-based binding screen and structural analysis of the complex formed between alpha-cobratoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica., Zeng H, Hawrot E, J Biol Chem. 2002 Oct 4;277(40):37439-45. Epub 2002 Jul 19. PMID:12133834
Page seeded by OCA on Sun Mar 30 22:08:36 2008
