This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4bw0

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 05:33, 5 August 2016

The molecular recognition of kink turn structure by the L7Ae class of proteins

Structural highlights

4bw0 is a 2 chain structure with sequence from Arcfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4c40
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RL7A_ARCFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.^[1]

Publication Abstract from PubMed

L7Ae is a member of a protein family that binds kink-turns (k-turns) in many functional RNA species. We have solved the X-ray crystal structure of the near-consensus sequence Kt-7 of Haloarcula marismortui bound by Archaeoglobus fulgidus L7Ae at 2.3-A resolution. We also present a structure of Kt-7 in the absence of bound protein at 2.2-A resolution. As a result, we can describe a general mode of recognition of k-turn structure by the L7Ae family proteins. The protein makes interactions in the widened major groove on the outer face of the k-turn. Two regions of the protein are involved. One is an alpha-helix that enters the major groove of the NC helix, making both nonspecific backbone interactions and specific interactions with the guanine nucleobases of the conserved G*A pairs. A hydrophobic loop makes close contact with the L1 and L2 bases, and a glutamate side chain hydrogen bonds with L1. Taken together, these interactions are highly selective for the structure of the k-turn and suggest how conformational selection of the folded k-turn occurs.

The molecular recognition of kink-turn structure by the L7Ae class of proteins.,Huang L, Lilley DM RNA. 2013 Oct 22. PMID:24149842^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rozhdestvensky TS, Tang TH, Tchirkova IV, Brosius J, Bachellerie JP, Huttenhofer A. Binding of L7Ae protein to the K-turn of archaeal snoRNAs: a shared RNA binding motif for C/D and H/ACA box snoRNAs in Archaea. Nucleic Acids Res. 2003 Feb 1;31(3):869-77. PMID:12560482
↑ Huang L, Lilley DM. The molecular recognition of kink-turn structure by the L7Ae class of proteins. RNA. 2013 Oct 22. PMID:24149842 doi:http://dx.doi.org/10.1261/rna.041517.113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bw0"

Categories: Arcfl | Huang, L | Lilley, D M.J | Rna-rna binding protein complex

@@ Line 1: / Line 1: @@
 ==The molecular recognition of kink turn structure by the L7Ae class of proteins==
 <StructureSection load='4bw0' size='340' side='right' caption='[[4bw0]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c40|4c40]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bw0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bw0 OCA], [http://pdbe.org/4bw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bw0 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4bw0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4bw0

From Proteopedia

Revision as of 05:33, 5 August 2016

The molecular recognition of kink turn structure by the L7Ae class of proteins

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox