2v5u

From Proteopedia

(Difference between revisions)

Revision as of 05:44, 5 August 2016

I92A FLAVODOXIN FROM ANABAENA

Structural highlights

2v5u is a 2 chain structure with sequence from Anabaena pcc7119. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1dx9, 1ftg, 1obo, 1obv, 1qhe, 2v5v
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FLAV_NOSSO] Low-potential electron donor to a number of redox enzymes.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Contribution of three regions (phosphate-binding, 50's and 90's loops) of Anabaena apoflavodoxin to FMN binding and reduction potential was studied. Thr12 and Glu16 did not influence FMN redox properties, but Thr12 played a role in FMN binding. Replacement of Trp57 with Glu, Lys or Arg moderately shifted E(ox/sq) and E(sq/hq) and altered the energetic of the FMN redox states binding profile. Our data indicate that the side chain of position 57 does not modulate E(ox/sq) by aromatic stacking or solvent exclusion, but rather by influencing the relative strength of the H-bond between the N(5) of the flavin and the Asn58-Ile59 bond. A correlation was observed between the isoalloxazine increase in solvent accessibility and less negative E(sq/hq). Moreover, E(sq/hq) became less negative as positively charged residues were added near to the isoalloxazine. Ile59 and Ile92 were simultaneously mutated to Ala or Glu. These mutations impaired FMN binding, while shifting E(sq/hq) to less negative values and E(ox/sq) to more negative. These effects are discussed on the bases of the X-ray structures of some of the Fld mutants, suggesting that in Anabaena Fld the structural control of both electron transfer steps is much more subtle than in other Flds.

Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin.,Frago S, Goni G, Herguedas B, Peregrina JR, Serrano A, Perez-Dorado I, Molina R, Gomez-Moreno C, Hermoso JA, Martinez-Julvez M, Mayhew SG, Medina M Arch Biochem Biophys. 2007 Nov 15;467(2):206-17. Epub 2007 Aug 29. PMID:17904516^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Frago S, Goni G, Herguedas B, Peregrina JR, Serrano A, Perez-Dorado I, Molina R, Gomez-Moreno C, Hermoso JA, Martinez-Julvez M, Mayhew SG, Medina M. Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin. Arch Biochem Biophys. 2007 Nov 15;467(2):206-17. Epub 2007 Aug 29. PMID:17904516 doi:10.1016/j.abb.2007.08.024

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v5u"

@@ Line 1: / Line 1: @@
 ==I92A FLAVODOXIN FROM ANABAENA==
 <StructureSection load='2v5u' size='340' side='right' caption='[[2v5u]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v5u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v5u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5U FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dx9|1dx9]], [[1ftg|1ftg]], [[1obo|1obo]], [[1obv|1obv]], [[1qhe|1qhe]], [[2v5v|2v5v]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v5u RCSB], [http://www.ebi.ac.uk/pdbsum/2v5u PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5u OCA], [http://pdbe.org/2v5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v5u RCSB], [http://www.ebi.ac.uk/pdbsum/2v5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5u ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FLAV_ANASO FLAV_ANASO]] Low-potential electron donor to a number of redox enzymes.
+[[http://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 17: / Line 18: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v5u ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 27: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2v5u" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 34: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Anabaena sp]]
+[[Category: Anabaena pcc7119]]
 [[Category: Frago, S]]
 [[Category: Hamiaux, C]]

2v5u

From Proteopedia

Revision as of 05:44, 5 August 2016

I92A FLAVODOXIN FROM ANABAENA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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