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3d65
From Proteopedia
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==Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom, in complex with trypsin== | ==Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom, in complex with trypsin== | ||
<StructureSection load='3d65' size='340' side='right' caption='[[3d65]], [[Resolution|resolution]] 1.64Å' scene=''> | <StructureSection load='3d65' size='340' side='right' caption='[[3d65]], [[Resolution|resolution]] 1.64Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3d65]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3d65]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Eastern_brown_snake Eastern brown snake]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D65 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3byb|3byb]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3byb|3byb]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d65 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3d65 RCSB], [http://www.ebi.ac.uk/pdbsum/3d65 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d65 OCA], [http://pdbe.org/3d65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d65 RCSB], [http://www.ebi.ac.uk/pdbsum/3d65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d65 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[http://www.uniprot.org/uniprot/ | + | [[http://www.uniprot.org/uniprot/VKT1_PSETT VKT1_PSETT]] Strongly inhibits plasmin (Ki=0.44 nM) and trypsin (Ki=0.42 nM). Has little effect on plasma (Ki=1870 nM) and tissue (Ki=12900 nM) kallikreins. In vivo, reduces bleeding in a small animal model.<ref>PMID:10847427</ref> <ref>PMID:12406072</ref> <ref>PMID:16707925</ref> <ref>PMID:19236611</ref> <ref>PMID:21843588</ref> <ref>PMID:23335990</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d65 ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bovin]] |
| - | [[Category: | + | [[Category: Eastern brown snake]] |
[[Category: Trypsin]] | [[Category: Trypsin]] | ||
[[Category: Guddat, L W]] | [[Category: Guddat, L W]] | ||
Revision as of 06:24, 5 August 2016
Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom, in complex with trypsin
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Categories: Bovin | Eastern brown snake | Trypsin | Guddat, L W | Jersey, J de | Lavin, M F | Masci, P P | Millers, E K.I | Blood | Coagulation | Digestion | Hydrolase | Hydrolase inhibitor-hydrolase complex | Metal-binding | Protease | Secreted | Serine protease | Serine protease inhibitor | Zymogen
