1m5o
From Proteopedia
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|PDB= 1m5o |SIZE=350|CAPTION= <scene name='initialview01'>1m5o</scene>, resolution 2.20Å | |PDB= 1m5o |SIZE=350|CAPTION= <scene name='initialview01'>1m5o</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=AVC:ADENOSINE-5'-MONOPHOSPHATE-2',3'-VANADATE'>AVC</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SNRPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= SNRPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1m5k|1M5K]], [[1m5p|1M5P]], [[1m5v|1M5V]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5o OCA], [http://www.ebi.ac.uk/pdbsum/1m5o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m5o RCSB]</span> | ||
}} | }} | ||
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[[Category: Rupert, P B.]] | [[Category: Rupert, P B.]] | ||
[[Category: Sigurdsson, S T.]] | [[Category: Sigurdsson, S T.]] | ||
| - | [[Category: CA]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
[[Category: hairpin ribozyme]] | [[Category: hairpin ribozyme]] | ||
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[[Category: vandate]] | [[Category: vandate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:11:36 2008'' |
Revision as of 19:11, 30 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | SNRPA (Homo sapiens) | ||||||
| Related: | 1M5K, 1M5P, 1M5V
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Transition State Stabilization by a Catalytic RNA
Overview
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes.
About this Structure
1M5O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Transition state stabilization by a catalytic RNA., Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR, Science. 2002 Nov 15;298(5597):1421-4. Epub 2002 Oct 10. PMID:12376595
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