1m5q
From Proteopedia
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|PDB= 1m5q |SIZE=350|CAPTION= <scene name='initialview01'>1m5q</scene>, resolution 2.00Å | |PDB= 1m5q |SIZE=350|CAPTION= <scene name='initialview01'>1m5q</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SmAP3 gene (Pae2122) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13773 Pyrobaculum aerophilum]) | |GENE= SmAP3 gene (Pae2122) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13773 Pyrobaculum aerophilum]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1i8f|1I8F]], [[1jbm|1JBM]], [[1i4k|1I4K]], [[1d3b|1D3B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5q OCA], [http://www.ebi.ac.uk/pdbsum/1m5q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m5q RCSB]</span> | ||
}} | }} | ||
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[[Category: Mura, C.]] | [[Category: Mura, C.]] | ||
[[Category: Phillips, M.]] | [[Category: Phillips, M.]] | ||
| - | [[Category: ACY]] | ||
| - | [[Category: CD]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: NA]] | ||
[[Category: 14-mer]] | [[Category: 14-mer]] | ||
[[Category: b-sheet toroid]] | [[Category: b-sheet toroid]] | ||
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[[Category: ob-like fold]] | [[Category: ob-like fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:11:38 2008'' |
Revision as of 19:11, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | SmAP3 gene (Pae2122) (Pyrobaculum aerophilum) | ||||||
| Related: | 1I8F, 1JBM, 1I4K, 1D3B
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a novel Sm-like archaeal protein from Pyrobaculum aerophilum
Overview
To better understand the roles of Sm proteins in forming the cores of many RNA-processing ribonucleoproteins, we determined the crystal structure of an atypical Sm-like archaeal protein (SmAP3) in which the conserved Sm domain is augmented by a previously uncharacterized, mixed alpha/beta C-terminal domain. The structure reveals an unexpected SmAP3 14-mer that is perforated by a cylindrical pore and is bound to 14 cadmium (Cd(2+)) ions. Individual heptamers adopt either "apical" or "equatorial" conformations that chelate Cd(2+) differently. SmAP3 forms supraheptameric oligomers (SmAP3)(n = 7,14,28) in solution, and assembly of the asymmetric 14-mer is modulated by differential divalent cation-binding in apical and equatorial subunits. Phylogenetic and sequence analyses substantiate SmAP3s as a unique subset of SmAPs. These results distinguish SmAP3s from other Sm proteins and provide a model for the structure and properties of Sm proteins >100 residues in length, e.g., several human Sm proteins.
About this Structure
1M5Q is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
Structure and assembly of an augmented Sm-like archaeal protein 14-mer., Mura C, Phillips M, Kozhukhovsky A, Eisenberg D, Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4539-44. Epub 2003 Mar 31. PMID:12668760
Page seeded by OCA on Sun Mar 30 22:11:38 2008
