1m60
From Proteopedia
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|PDB= 1m60 |SIZE=350|CAPTION= <scene name='initialview01'>1m60</scene> | |PDB= 1m60 |SIZE=350|CAPTION= <scene name='initialview01'>1m60</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=HES:ZINC SUBSTITUTED HEME C'>HES</scene> | + | |LIGAND= <scene name='pdbligand=HES:ZINC+SUBSTITUTED+HEME+C'>HES</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m60 OCA], [http://www.ebi.ac.uk/pdbsum/1m60 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m60 RCSB]</span> | ||
}} | }} | ||
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[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: Yao, Y.]] | [[Category: Yao, Y.]] | ||
| - | [[Category: HES]] | ||
[[Category: six-coordinated zinc cyt c]] | [[Category: six-coordinated zinc cyt c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:11:47 2008'' |
Revision as of 19:11, 30 March 2008
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Zinc-substituted cytochrome c
Overview
Zinc-substituted cytochrome c has been widely used in studies of protein-protein interactions and photo-induced electron transfer reactions between proteins. However, the coordination geometry of zinc in zinc-substituted cyt c has not yet been determined; two different opinions about the coordination have been reached. Here the solution structures of zinc-substituted cytochrome c that might be five-coordinated and six-coordinated have been refined separately by using (1)H NMR spectroscopy, and the zinc coordination geometry was determined just by NOE distance constraints. Structural analysis of the energy-minimized average solution structures of both the pentacoordinated and hexacoordinated geometries indicate that that zinc in zinc-substituted cyt c should be bound to both His18 and Met80, which means that the zinc is six-coordinated. RMSD values of the family of 25 six-coordinated structures from the average structure are 0.66+/-0.13 A and 1.09+/-0.16 A for the backbone and all heavy atoms, respectively. A statistical analysis of the structure indicates its satisfactory quality. Comparison of the solution structure of the six-coordinated energy-minimized average structure of zinc-substituted cytochrome c with the solution structure of reduced cytochrome c reveals that for the overall folding the secondary structure elements are very close. The availability of the structure provides for a better understanding of the protein-protein complex and for electron transfer processes between Zn cyt c and other metalloproteins.
About this Structure
1M60 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Structural analysis of zinc-substituted cytochrome c., Qian C, Yao Y, Tong Y, Wang J, Tang W, J Biol Inorg Chem. 2003 Apr;8(4):394-400. Epub 2002 Dec 14. PMID:12761660
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