2yil

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(Difference between revisions)

Revision as of 09:08, 5 August 2016

CRYSTAL STRUCTURE OF PARASITE SARCOCYSTIS MURIS LECTIN SML-2

Structural highlights

2yil is a 6 chain structure with sequence from Sarcocystis muris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	2yio, 2yip
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MIA2_SARMU] Galactose-binding lectin. Plays a role in adhesion to the host cell. Has a potential role in invasion of host cells.^[1] ^[2]

Publication Abstract from PubMed

The microneme protein SML-2 is a member of a small family of galactose-specific lectins that play a role during host-cell invasion by the apicomplexan parasite Sarcocystis muris. The structures of apo SML-2 and the 1-thio-beta-D-galactose-SML-2 complex were determined at 1.95 and 2.1 A resolution, respectively, by sulfur-SAD phasing. Highly elongated dimers are formed by PAN-domain tandems in the protomer, bearing the galactose-binding cavities at the distal apple-like domains. The detailed structure of the binding site in SML-2 explains the high specificity of galactose-endgroup binding and the broader specificity of the related Toxoplasma gondii protein TgMIC4 towards galactose and glucose. A large buried surface of highly hydrophobic character and 24 intersubunit hydrogen bonds stabilize the dimers and half of the 12 disulfides per dimer are shielded from the solvent by the polypeptide chain, thereby enhancing the resistance of the parasite protein towards unfolding and proteolysis that allows it to survive within the intestinal tracts of the intermediate and final hosts.

PAN-modular structure of microneme protein SML-2 from the parasite Sarcocystis muris at 1.95 A resolution and its complex with 1-thio-beta-D-galactose.,Muller JJ, Weiss MS, Heinemann U Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):936-44. Epub 2011, Oct 19. PMID:22101820^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Klein H, Mueller S, Loeschner B, Toenjes RR, Braun G, Mueller EC, Otto A, Montag T. Cloning, sequencing and recombinant expression of the open reading frame encoding a novel member of the Sarcocystis muris (Apicomplexa) microneme lectin family. Parasitol Res. 2003 May;90(1):84-6. Epub 2003 Feb 14. PMID:12743810 doi:http://dx.doi.org/10.1007/s00436-002-0600-0
↑ Muller JJ, Weiss MS, Heinemann U. PAN-modular structure of microneme protein SML-2 from the parasite Sarcocystis muris at 1.95 A resolution and its complex with 1-thio-beta-D-galactose. Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):936-44. Epub 2011, Oct 19. PMID:22101820 doi:10.1107/S0907444911037796
↑ Muller JJ, Weiss MS, Heinemann U. PAN-modular structure of microneme protein SML-2 from the parasite Sarcocystis muris at 1.95 A resolution and its complex with 1-thio-beta-D-galactose. Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):936-44. Epub 2011, Oct 19. PMID:22101820 doi:10.1107/S0907444911037796

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yil"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF PARASITE SARCOCYSTIS MURIS LECTIN SML-2==
 <StructureSection load='2yil' size='340' side='right' caption='[[2yil]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yio|2yio]], [[2yip|2yip]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yil RCSB], [http://www.ebi.ac.uk/pdbsum/2yil PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yil OCA], [http://pdbe.org/2yil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yil RCSB], [http://www.ebi.ac.uk/pdbsum/2yil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yil ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/MIA2_SARMU MIA2_SARMU]] Galactose-binding lectin. Plays a role in adhesion to the host cell. Has a potential role in invasion of host cells.<ref>PMID:12743810</ref> <ref>PMID:22101820</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2yil" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

2yil

From Proteopedia

Revision as of 09:08, 5 August 2016

CRYSTAL STRUCTURE OF PARASITE SARCOCYSTIS MURIS LECTIN SML-2

Structural highlights

Function

Publication Abstract from PubMed

References

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