1ma8
From Proteopedia
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|PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3Å | |PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=UMS:2'-METHYLSELENYL-2'-DEOXYURIDINE-5'-PHOSPHATE'>UMS</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [http://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB]</span> | ||
}} | }} | ||
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[[Category: 2'-methylseleno-uridine]] | [[Category: 2'-methylseleno-uridine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:26 2008'' |
Revision as of 19:13, 30 March 2008
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| , resolution 1.3Å | |||||||
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| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
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