4ghq

From Proteopedia

(Difference between revisions)

Revision as of 10:01, 5 August 2016

Crystal structure of EV71 3C proteinase

Structural highlights

4ghq is a 1 chain structure with sequence from Ev-71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4ght, 4ghv, 4ghx, 4ghy, 4ghz
Activity:	Picornain 3C, with EC number 3.4.22.28
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A9XG43_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

The crystal structure of 3C proteinase (3C(pro)) from Enterovirus 71 (EV71) was determined in space group C2221 to 2.2 A resolution. The fold was similar to that of 3C(pro) from other picornaviruses, but the difference in the beta-ribbon reported in a previous structure was not observed. This beta-ribbon was folded over the substrate-binding cleft and constituted part of the essential binding sites for interaction with the substrate. The structure of its complex with rupintrivir (AG7088), a peptidomimetic inhibitor, was also characterized in space group P212121 to 1.96 A resolution. The inhibitor was accommodated without any spatial hindrance despite the more constricted binding site; this was confirmed by functional assays, in which the inhibitor showed comparable potency towards EV71 3C(pro) and human rhinovirus 3C(pro), which is the target that rupintrivir was designed against.

Structures of Enterovirus 71 3C proteinase (strain E2004104-TW-CDC) and its complex with rupintrivir.,Wu C, Cai Q, Chen C, Li N, Peng X, Cai Y, Yin K, Chen X, Wang X, Zhang R, Liu L, Chen S, Li J, Lin T Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):866-71. doi:, 10.1107/S0907444913002862. Epub 2013 Apr 19. PMID:23633597^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wu C, Cai Q, Chen C, Li N, Peng X, Cai Y, Yin K, Chen X, Wang X, Zhang R, Liu L, Chen S, Li J, Lin T. Structures of Enterovirus 71 3C proteinase (strain E2004104-TW-CDC) and its complex with rupintrivir. Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):866-71. doi:, 10.1107/S0907444913002862. Epub 2013 Apr 19. PMID:23633597 doi:10.1107/S0907444913002862

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ghq"

@@ Line 1: / Line 1: @@
 ==Crystal structure of EV71 3C proteinase==
 <StructureSection load='4ghq' size='340' side='right' caption='[[4ghq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ghq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterovirus_a71 Enterovirus a71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GHQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ghq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GHQ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ght|4ght]], [[4ghv|4ghv]], [[4ghx|4ghx]], [[4ghy|4ghy]], [[4ghz|4ghz]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_3C Picornain 3C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.28 3.4.22.28] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ghq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ghq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ghq RCSB], [http://www.ebi.ac.uk/pdbsum/4ghq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ghq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ghq OCA], [http://pdbe.org/4ghq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ghq RCSB], [http://www.ebi.ac.uk/pdbsum/4ghq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ghq ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4ghq" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Proteinase|Proteinase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Enterovirus a71]]
+[[Category: Ev-71]]
 [[Category: Picornain 3C]]
 [[Category: Cai, Q]]

4ghq

From Proteopedia

Revision as of 10:01, 5 August 2016

Crystal structure of EV71 3C proteinase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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