4u1e

From Proteopedia

(Difference between revisions)

Revision as of 11:08, 5 August 2016

Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex

Structural highlights

4u1e is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4u1f, 4u1d, 4u1c
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EIF3I_YEAS7] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome (By similarity). [EIF3G_YEAST] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. TIF35 binds to mRNA and rRNA. Binds to the 18S rRNA but non-specifically.^[1] [EIF3B_YEAST] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.^[2]

Publication Abstract from PubMed

Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40SeIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.

Molecular Architecture of the 40SeIF1eIF3 Translation Initiation Complex.,Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CH, Cimermancic P, Boehringer D, Sali A, Aebersold R, Ban N Cell. 2014 Aug 28;158(5):1123-35. doi: 10.1016/j.cell.2014.07.044. PMID:25171412^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hanachi P, Hershey JW, Vornlocher HP. Characterization of the p33 subunit of eukaryotic translation initiation factor-3 from Saccharomyces cerevisiae. J Biol Chem. 1999 Mar 26;274(13):8546-53. PMID:10085088
↑ Phan L, Schoenfeld LW, Valasek L, Nielsen KH, Hinnebusch AG. A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J. 2001 Jun 1;20(11):2954-65. PMID:11387228 doi:http://dx.doi.org/10.1093/emboj/20.11.2954
↑ Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CH, Cimermancic P, Boehringer D, Sali A, Aebersold R, Ban N. Molecular Architecture of the 40SeIF1eIF3 Translation Initiation Complex. Cell. 2014 Aug 28;158(5):1123-35. doi: 10.1016/j.cell.2014.07.044. PMID:25171412 doi:http://dx.doi.org/10.1016/j.cell.2014.07.044

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4u1e"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex==
 <StructureSection load='4u1e' size='340' side='right' caption='[[4u1e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4u1e]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U1E FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4u1f|4u1f]], [[4u1d|4u1d]], [[4u1c|4u1c]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u1e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u1e RCSB], [http://www.ebi.ac.uk/pdbsum/4u1e PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u1e OCA], [http://pdbe.org/4u1e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u1e RCSB], [http://www.ebi.ac.uk/pdbsum/4u1e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u1e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4u1e" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
 == References ==
 <references/>

4u1e

From Proteopedia

Revision as of 11:08, 5 August 2016

Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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