1meg
From Proteopedia
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|PDB= 1meg |SIZE=350|CAPTION= <scene name='initialview01'>1meg</scene>, resolution 2.0Å | |PDB= 1meg |SIZE=350|CAPTION= <scene name='initialview01'>1meg</scene>, resolution 2.0Å | ||
|SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene> | |SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene> | ||
| - | |LIGAND= <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene> | + | |LIGAND= <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Caricain Caricain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.30 3.4.22.30] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Caricain Caricain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.30 3.4.22.30] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1meg OCA], [http://www.ebi.ac.uk/pdbsum/1meg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1meg RCSB]</span> | ||
}} | }} | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Katerelos, N A.]] | [[Category: Katerelos, N A.]] | ||
| - | [[Category: E64]] | ||
| - | [[Category: EOH]] | ||
[[Category: cysteine proteinase]] | [[Category: cysteine proteinase]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: thiol protease]] | [[Category: thiol protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:15:06 2008'' |
Revision as of 19:15, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Activity: | Caricain, with EC number 3.4.22.30 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64
Overview
The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was not anticipated since in the native structure there is insufficient room to accommodate the glutamate side chain. The glutamate is accommodated in the mutant by a local expansion of the structure demonstrating that small structural changes are responsible for the change in activity.
About this Structure
1MEG is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.
Reference
Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:8769310
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