4koo

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Revision as of 11:34, 5 August 2016

Crystal Structure of WHY1 from Arabidopsis thaliana

Structural highlights

4koo is a 4 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4kop, 4koq
Gene:	At1g14410, F14L17.18, PTAC1, WHY1 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[WHY1_ARATH] Single-stranded DNA-binding protein that functions in both chloroplasts and nucleus. In chloroplasts, maintains plastid genome stability by preventing break-induced and short homology-dependent illegitimate recombinations. In nucleus, modulates telomere length homeostasis by inhibiting the action of the telomerase at the extreme termini of chromosomes. Is recruited to a distal element upstream of the kinesin KP1 to mediate the transcriptional repression of KP1. Is required for full salicylic acid-dependent plant disease resistance responses. Can bind double-stranded DNA in vivo.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

DNA double-strand breaks are highly detrimental genomic lesions that routinely arise in genomes. To protect the integrity of their genetic information, all organisms have evolved specialized DNA-repair mechanisms. Whirly proteins modulate DNA repair in plant chloroplasts and mitochondria by binding single-stranded DNA in a non-sequence-specific manner. Although most of the results showing the involvement of the Whirly proteins in DNA repair have been obtained in Arabidopsis thaliana, only the crystal structures of the potato Whirly proteins WHY1 and WHY2 have been reported to date. The present report of the crystal structures of the three Whirly proteins from A. thaliana (WHY1, WHY2 and WHY3) reveals that these structurally similar proteins assemble into tetramers. Furthermore, structural alignment with a potato WHY2-DNA complex reveals that the residues in these proteins are properly oriented to bind single-stranded DNA in a non-sequence-specific manner.

A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum.,Cappadocia L, Parent JS, Sygusch J, Brisson N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1207-11., doi: 10.1107/S1744309113028698. Epub 2013 Oct 26. PMID:24192350^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Desveaux D, Subramaniam R, Despres C, Mess JN, Levesque C, Fobert PR, Dangl JL, Brisson N. A "Whirly" transcription factor is required for salicylic acid-dependent disease resistance in Arabidopsis. Dev Cell. 2004 Feb;6(2):229-40. PMID:14960277
↑ Yoo HH, Kwon C, Lee MM, Chung IK. Single-stranded DNA binding factor AtWHY1 modulates telomere length homeostasis in Arabidopsis. Plant J. 2007 Feb;49(3):442-51. Epub 2007 Jan 1. PMID:17217467 doi:http://dx.doi.org/10.1111/j.1365-313X.2006.02974.x
↑ Xiong JY, Lai CX, Qu Z, Yang XY, Qin XH, Liu GQ. Recruitment of AtWHY1 and AtWHY3 by a distal element upstream of the kinesin gene AtKP1 to mediate transcriptional repression. Plant Mol Biol. 2009 Nov;71(4-5):437-49. doi: 10.1007/s11103-009-9533-7. Epub, 2009 Aug 11. PMID:19669906 doi:http://dx.doi.org/10.1007/s11103-009-9533-7
↑ Marechal A, Parent JS, Veronneau-Lafortune F, Joyeux A, Lang BF, Brisson N. Whirly proteins maintain plastid genome stability in Arabidopsis. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14693-8. doi:, 10.1073/pnas.0901710106. Epub 2009 Aug 7. PMID:19666500 doi:http://dx.doi.org/10.1073/pnas.0901710106
↑ Cappadocia L, Marechal A, Parent JS, Lepage E, Sygusch J, Brisson N. Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair. Plant Cell. 2010 Jun;22(6):1849-67. Epub 2010 Jun 15. PMID:20551348 doi:10.1105/tpc.109.071399
↑ Cappadocia L, Parent JS, Zampini E, Lepage E, Sygusch J, Brisson N. A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage. Nucleic Acids Res. 2011 Sep 12. PMID:21911368 doi:10.1093/nar/gkr740
↑ Cappadocia L, Parent JS, Sygusch J, Brisson N. A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1207-11., doi: 10.1107/S1744309113028698. Epub 2013 Oct 26. PMID:24192350 doi:http://dx.doi.org/10.1107/S1744309113028698

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4koo"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of WHY1 from Arabidopsis thaliana==
 <StructureSection load='4koo' size='340' side='right' caption='[[4koo]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kop|4kop]], [[4koq|4koq]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At1g14410, F14L17.18, PTAC1, WHY1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4koo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4koo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4koo RCSB], [http://www.ebi.ac.uk/pdbsum/4koo PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4koo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4koo OCA], [http://pdbe.org/4koo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4koo RCSB], [http://www.ebi.ac.uk/pdbsum/4koo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4koo ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4koo" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4koo

From Proteopedia

Revision as of 11:34, 5 August 2016

Crystal Structure of WHY1 from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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