4diy
From Proteopedia
(Difference between revisions)
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| - | [[Image:4diy.png|left|200px]] | ||
| - | + | ==Thaumatin I by Classical Hanging Drop Method at 1.98A resolution for Unique Water Distribution== | |
| + | <StructureSection load='4diy' size='340' side='right' caption='[[4diy]], [[Resolution|resolution]] 1.98Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4diy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DIY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DIY FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4diz|4diz]], [[4dj0|4dj0]], [[4dj1|4dj1]], [[4dj5|4dj5]], [[3dzr|3dzr]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4diy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4diy OCA], [http://pdbe.org/4diy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4diy RCSB], [http://www.ebi.ac.uk/pdbsum/4diy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4diy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Langmuir-Blodgett films when used as nanotemplates for crystallization often leads to marked changes in protein stability and structure. Earlier we found that stability of proteins is also correlated with aqueous surroundings in the crystals. Here we study the direct relationships between presence of LB nanotemplates and unique patterns of water molecules surrounding the protein, for four model proteins for which 3D structures are available, and where crystallization conditions for each protein are the same except the presence of LB nanotemplate. Shape of frequency distribution of volumes occupied by water molecules were analyzed. They were found to be different between "classical" samples of different proteins, but surprisingly quite similar for LB samples. Volumes occupied by each water molecule as the function of the distance of the given molecule from the protein surface were studied. Introduction of LB film leads to appearance of water molecules close to protein surface but occupying large volumes. These findings confirm earlier experimental findings on the role of water molecules in determining protein stability and thereby pointing to water as a possible candidate for differences apparent in LB crystal stability against radiation. | ||
| - | + | Unique water distribution of Langmuir-Blodgett versus classical crystals.,Pechkova E, Sivozhelezov V, Belmonte L, Nicolini C J Struct Biol. 2012 Jun 15. PMID:22706161<ref>PMID:22706161</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4diy" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
[[Category: Thaumatococcus daniellii]] | [[Category: Thaumatococcus daniellii]] | ||
| - | [[Category: Belmonte, L | + | [[Category: Belmonte, L]] |
| - | [[Category: Nicolini, C | + | [[Category: Nicolini, C]] |
| - | [[Category: Pechkova, E | + | [[Category: Pechkova, E]] |
| - | [[Category: Sivozhelezov, V | + | [[Category: Sivozhelezov, V]] |
[[Category: Langmuir-blodgett]] | [[Category: Langmuir-blodgett]] | ||
[[Category: Plant protein]] | [[Category: Plant protein]] | ||
[[Category: Thin-film]] | [[Category: Thin-film]] | ||
[[Category: Water distribution]] | [[Category: Water distribution]] | ||
Revision as of 11:40, 5 August 2016
Thaumatin I by Classical Hanging Drop Method at 1.98A resolution for Unique Water Distribution
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