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3u1m

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Revision as of 12:13, 5 August 2016

Structure of the mRNA splicing complex component Cwc2

Structural highlights

3u1m is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3u1l
Gene:	CWC2, NTC40, SLC3, YDL209C, D1041 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CWC2_YEAST] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.^[1] ^[2]

Publication Abstract from PubMed

The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 A (1 A=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domains, a CCCH-type ZnF (zinc finger) and a RRM (RNA recognition motif). Unexpectedly, the ZnF domain and the RRM form a single folding unit, glued together by extensive hydrophobic interactions and hydrogen bonds. Structure-guided mutational analysis revealed that the intervening loop [known as the RB loop (RNA-binding loop)] between ZnF and RRM plays an essential role in RNA binding. In addition, a number of highly conserved positively charged residues on the beta-strands of RRM make an important contribution to RNA binding. Intriguingly, these residues and a portion of the RB loop constitute an extended basic surface strip that encircles Cwc2 halfway. The present study serves as a framework for understanding the regulatory function of the NTC in RNA splicing.

Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition.,Lu P, Lu G, Yan C, Wang L, Li W, Yin P Biochem J. 2012 Jan 15;441(2):591-7. doi: 10.1042/BJ20111385. PMID:21957909^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McGrail JC, Krause A, O'Keefe RT. The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res. 2009 Jul;37(13):4205-17. Epub 2009 May 12. PMID:19435883 doi:http://dx.doi.org/gkp341
↑ Rasche N, Dybkov O, Schmitzova J, Akyildiz B, Fabrizio P, Luhrmann R. Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO J. 2012 Mar 21;31(6):1591-604. doi: 10.1038/emboj.2011.502. Epub 2012 Jan, 13. PMID:22246180 doi:http://dx.doi.org/10.1038/emboj.2011.502
↑ Lu P, Lu G, Yan C, Wang L, Li W, Yin P. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. 2012 Jan 15;441(2):591-7. doi: 10.1042/BJ20111385. PMID:21957909 doi:http://dx.doi.org/10.1042/BJ20111385

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3u1m"

@@ Line 1: / Line 1: @@
 ==Structure of the mRNA splicing complex component Cwc2==
 <StructureSection load='3u1m' size='340' side='right' caption='[[3u1m]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u1l|3u1l]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CWC2, NTC40, SLC3, YDL209C, D1041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u1m RCSB], [http://www.ebi.ac.uk/pdbsum/3u1m PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u1m OCA], [http://pdbe.org/3u1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u1m RCSB], [http://www.ebi.ac.uk/pdbsum/3u1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u1m ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3u1m" style="background-color:#fffaf0;"></div>
 ==See Also==

3u1m

From Proteopedia

Revision as of 12:13, 5 August 2016

Structure of the mRNA splicing complex component Cwc2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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