3zvl

From Proteopedia

(Difference between revisions)

Revision as of 12:35, 5 August 2016

THE STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY MAMMALIAN POLYNUCLEOTIDE KINASE 3' PHOSPHATASE

Structural highlights

3zvl is a 1 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
NonStd Res:
Related:	1ujx, 1yj5, 1yjm
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PNKP_MOUSE] Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

Publication Abstract from PubMed

Mammalian polynucleotide kinase 3' phosphatase (PNK) plays a key role in the repair of DNA damage, functioning as part of both the nonhomologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. We have now determined crystal structures of murine PNK with DNA molecules bound to both of its active sites. The structure of ssDNA engaged with the 3'-phosphatase domain suggests a mechanism of substrate interaction that assists DNA end seeking. The structure of dsDNA bound to the 5'-kinase domain reveals a mechanism of DNA bending that facilitates recognition of DNA ends in the context of single-strand and double-strand breaks and suggests a close functional cooperation in substrate recognition between the kinase and phosphatase active sites.

The structural basis for substrate recognition by Mammalian polynucleotide kinase 3' phosphatase.,Garces F, Pearl LH, Oliver AW Mol Cell. 2011 Nov 4;44(3):385-96. PMID:22055185^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garces F, Pearl LH, Oliver AW. The structural basis for substrate recognition by Mammalian polynucleotide kinase 3' phosphatase. Mol Cell. 2011 Nov 4;44(3):385-96. PMID:22055185 doi:10.1016/j.molcel.2011.08.036

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zvl"

@@ Line 1: / Line 1: @@
 ==THE STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY MAMMALIAN POLYNUCLEOTIDE KINASE 3' PHOSPHATASE==
 <StructureSection load='3zvl' size='340' side='right' caption='[[3zvl]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zvl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zvl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZVL FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ujx|1ujx]], [[1yj5|1yj5]], [[1yjm|1yjm]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zvl RCSB], [http://www.ebi.ac.uk/pdbsum/3zvl PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvl OCA], [http://pdbe.org/3zvl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zvl RCSB], [http://www.ebi.ac.uk/pdbsum/3zvl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zvl" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Mus musculus]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Garces, F]]
 [[Category: Oliver, A W]]

3zvl

From Proteopedia

Revision as of 12:35, 5 August 2016

THE STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION BY MAMMALIAN POLYNUCLEOTIDE KINASE 3' PHOSPHATASE

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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