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4cew

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Revision as of 12:53, 5 August 2016

Crystal structure of human Enterovirus 71 in complex with the uncoating inhibitor ALD

Structural highlights

4cew is a 4 chain structure with sequence from Ev-71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4cdq, 4cdu, 4cdw, 4cdx, 4cey
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[B2ZUN0_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

Enterovirus 71 (HEV71) epidemics in children and infants result mainly in mild symptoms; however, especially in the Asia-Pacific region, infection can be fatal. At present, no therapies are available. We have used structural analysis of the complete virus to guide the design of HEV71 inhibitors. Analysis of complexes with four 3-(4-pyridyl)-2-imidazolidinone derivatives with varying anti-HEV71 activities pinpointed key structure-activity correlates. We then identified additional potentially beneficial substitutions, developed methods to reliably triage compounds by quantum mechanics-enhanced ligand docking and synthesized two candidates. Structural analysis and in vitro assays confirmed the predicted binding modes and their ability to block viral infection. One ligand (with IC50 of 25 pM) is an order of magnitude more potent than the best previously reported inhibitor and is also more soluble. Our approach may be useful in the design of effective drugs for enterovirus infections.

More-powerful virus inhibitors from structure-based analysis of HEV71 capsid-binding molecules.,De Colibus L, Wang X, Spyrou JA, Kelly J, Ren J, Grimes J, Puerstinger G, Stonehouse N, Walter TS, Hu Z, Wang J, Li X, Peng W, Rowlands DJ, Fry EE, Rao Z, Stuart DI Nat Struct Mol Biol. 2014 Feb 9. doi: 10.1038/nsmb.2769. PMID:24509833^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ De Colibus L, Wang X, Spyrou JA, Kelly J, Ren J, Grimes J, Puerstinger G, Stonehouse N, Walter TS, Hu Z, Wang J, Li X, Peng W, Rowlands DJ, Fry EE, Rao Z, Stuart DI. More-powerful virus inhibitors from structure-based analysis of HEV71 capsid-binding molecules. Nat Struct Mol Biol. 2014 Feb 9. doi: 10.1038/nsmb.2769. PMID:24509833 doi:http://dx.doi.org/10.1038/nsmb.2769

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cew"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human Enterovirus 71 in complex with the uncoating inhibitor ALD==
 <StructureSection load='4cew' size='340' side='right' caption='[[4cew]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7J5:4-[3-[(3S)-5-[4-[(E)-ETHOXYIMINOMETHYL]PHENOXY]-3-METHYL-PENTYL]-2-OXIDANYLIDENE-IMIDAZOLIDIN-1-YL]PYRIDINE-2-CARBOXAMIDE'>7J5</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cdq|4cdq]], [[4cdu|4cdu]], [[4cdw|4cdw]], [[4cdx|4cdx]], [[4cey|4cey]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cew OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cew RCSB], [http://www.ebi.ac.uk/pdbsum/4cew PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cew OCA], [http://pdbe.org/4cew PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cew RCSB], [http://www.ebi.ac.uk/pdbsum/4cew PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cew ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4cew" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4cew

From Proteopedia

Revision as of 12:53, 5 August 2016

Crystal structure of human Enterovirus 71 in complex with the uncoating inhibitor ALD

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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