3uit

From Proteopedia

(Difference between revisions)

Revision as of 12:59, 5 August 2016

Overall structure of Patj/Pals1/Mals complex

Structural highlights

3uit is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[INADL_MOUSE] Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane. May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons.^[1]

Publication Abstract from PubMed

The assembly of supramolecular complexes in multidomain scaffold proteins is crucial for the control of cell polarity. The scaffold protein of protein associated with Lin-7 1 (Pals1) forms a complex with two other scaffold proteins, Pals-associated tight junction protein (Patj) and mammalian homolog-2 of Lin-7 (Mals2), through its tandem Lin-2 and Lin-7 (L27) domains to regulate apical-basal polarity. Here, we report the crystal structure of a 4-L27 domain-containing heterotrimer derived from the tripartite complex Patj/Pals1/Mals2. The heterotrimer consists of two cognate pairs of heterodimeric L27 domains with similar conformations. Structural analysis and biochemical data further show that the dimers assemble mutually independently. Additionally, such mutually independent assembly of the two heterodimers can be observed in another tripartite complex, Disks large homolog 1 (DLG1)/calcium-calmodulin-dependent serine protein kinase (CASK)/Mals2. Our results reveal a novel mechanism for tandem L27 domain-mediated, supramolecular complex assembly with a mutually independent mode.

Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode.,Zhang J, Yang X, Wang Z, Zhou H, Xie X, Shen Y, Long J J Biol Chem. 2012 Mar 30;287(14):11132-40. Epub 2012 Feb 15. PMID:22337881^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Anzai N, Deval E, Schaefer L, Friend V, Lazdunski M, Lingueglia E. The multivalent PDZ domain-containing protein CIPP is a partner of acid-sensing ion channel 3 in sensory neurons. J Biol Chem. 2002 May 10;277(19):16655-61. Epub 2002 Feb 28. PMID:11872753 doi:10.1074/jbc.M201087200
↑ Zhang J, Yang X, Wang Z, Zhou H, Xie X, Shen Y, Long J. Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode. J Biol Chem. 2012 Mar 30;287(14):11132-40. Epub 2012 Feb 15. PMID:22337881 doi:10.1074/jbc.M111.321216

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3uit"

@@ Line 1: / Line 1: @@
 ==Overall structure of Patj/Pals1/Mals complex==
 <StructureSection load='3uit' size='340' side='right' caption='[[3uit]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3uit]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UIT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3uit]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UIT FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uit RCSB], [http://www.ebi.ac.uk/pdbsum/3uit PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uit OCA], [http://pdbe.org/3uit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uit RCSB], [http://www.ebi.ac.uk/pdbsum/3uit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uit ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3uit" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Human]]
 [[Category: Long, J]]
 [[Category: Shen, Y]]

3uit

From Proteopedia

Revision as of 12:59, 5 August 2016

Overall structure of Patj/Pals1/Mals complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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