1mjg
From Proteopedia
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|PDB= 1mjg |SIZE=350|CAPTION= <scene name='initialview01'>1mjg</scene>, resolution 2.2Å | |PDB= 1mjg |SIZE=350|CAPTION= <scene name='initialview01'>1mjg</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=XCC:FE(4)-NI(1)-S(4)+CLUSTER'>XCC</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1jqk|1JQK]], [[1jjy|1JJY]], [[1e9v|1E9V]], [[1e2u|1E2U]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjg OCA], [http://www.ebi.ac.uk/pdbsum/1mjg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mjg RCSB]</span> | ||
}} | }} | ||
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[[Category: Ragsdale, S W.]] | [[Category: Ragsdale, S W.]] | ||
[[Category: Seravalli, J.]] | [[Category: Seravalli, J.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: CU1]] | ||
| - | [[Category: NI]] | ||
| - | [[Category: SF4]] | ||
| - | [[Category: XCC]] | ||
[[Category: acetyl-coa synthase (acs)]] | [[Category: acetyl-coa synthase (acs)]] | ||
[[Category: carbon monoxide dehydrogenase(codh)]] | [[Category: carbon monoxide dehydrogenase(codh)]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
[[Category: substrate tunnel]] | [[Category: substrate tunnel]] | ||
| - | [[Category: wood-ljundahl pathway]] | + | [[Category: wood-ljundahl pathway,]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:52 2008'' |
Revision as of 19:16, 30 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Activity: | Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2 | ||||||
| Related: | 1JQK, 1JJY, 1E9V, 1E2U
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)
Overview
A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.
About this Structure
1MJG is a Protein complex structure of sequences from Moorella thermoacetica. Full crystallographic information is available from OCA.
Reference
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:12386327
Page seeded by OCA on Sun Mar 30 22:16:52 2008
Categories: Carbon-monoxide dehydrogenase (acceptor) | Moorella thermoacetica | Protein complex | Doukov, T I. | Drennan, C L. | Iverson, T M. | Ragsdale, S W. | Seravalli, J. | Acetyl-coa synthase (acs) | Carbon monoxide dehydrogenase(codh) | Clostridium thermoaceticum | Electron transfer | Helical domain | Hydrophobic co channel | Nickel-coopper-iron-sulfur (ni-cu-fe-s) cluster | Nickel-iron-sulfur cluster (ni-fe-s) | Rossmann fold | Substrate tunnel | Wood-ljundahl pathway,
